iFluor™ 647 Conjugated Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]

Function

The sodium–potassium pump (sodium–potassium adenosine triphosphatase, also known as Na⁺/K⁺-ATPase, Na⁺/K⁺ pump, or sodium–potassium ATPase) is an enzyme (an electrogenic transmembrane ATPase) found in the membrane of all animal cells. It performs several functions in cell physiology. The Na⁺/K⁺-ATPase helps maintain resting potential, affects transport, and regulates cellular volume. It also functions as a signal transducer/integrator to regulate the MAPK pathway, reactive oxygen species (ROS), as well as intracellular calcium. In fact, all cells expend a large fraction of the ATP they produce (typically 30% and up to 70% in nerve cells) to maintain their required cytosolic Na and K concentrations. For neurons, the Na⁺/K⁺-ATPase can be responsible for up to 3/4 of the cell's energy expenditure. In many types of tissue, ATP consumption by the Na⁺/K⁺-ATPases have been related to glycolysis. This was first discovered in red blood cells (Schrier, 1966), but has later been evidenced in renal cells, smooth muscles surrounding the blood vessels,[6] and cardiac purkinje cells. Recently, glycolysis has also been shown to be of particular importance for Na⁺/K⁺-ATPases in skeletal muscles, where inhibition of glycogen breakdown (a substrate for glycolysis) leads to reduced Na⁺/K⁺-ATPase activity and lower force production.

Background References

1. Yang SH et al. The lamellae-free-type pseudobranch of the euryhaline milkfish (Chanos chanos) is a Na(+), K(+)-ATPase-abundant organ involved in hypoosmoregulation. Comp Biochem Physiol A Mol Integr Physiol 170:15-25 (2014).

2. R der PV et al. The role of SGLT1 and GLUT2 in intestinal glucose transport and sensing. PLoS One 9:e89977 (2014).

Sequence Similarity

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Post-translational Modification

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).

Subcellular Location

Cell membrane, Melanosome.

UNIPROT

SwissProt: P05023 Human

SwissProt: P05026 Human

SwissProt: Q13733 Human

SwissProt: P06685 Rat

SwissProt: P07340 Rat

SwissProt: Q64541 Rat

Synonyms