Skip to content

Src Recombinant Rabbit Monoclonal Antibody [ST05-03]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1609-65

Src Recombinant Rabbit Monoclonal Antibody [ST05-03]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Src Recombinant Rabbit Monoclonal Antibody [ST05-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Src aa 437-536 / 536.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Molecular Weight

Predicted band size: 60 kDa

Positive Control

A549 cell lysate, A431 cell lysate, Mouse cerebellum tissue lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat cerebellum tissue lysate, Rat lung tissue lysate, A431, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

ST05-03

RRID

AB_3069874

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

The major translational products of the Src gene family are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences. By virtue of their common structural motifs, the Src family is composed of nine members in vertebrates, including c-Src, c-Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases, which contain an amino-terminal cell membrane anchor followed by SH3 and SH2 domains, transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family members are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular locations of Src family kinases may be important for the regulation of specific cellular processes, such as mitogenesis, cytoskeletal organization and membrane trafficking. c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.

Background References

1. Villacis RA et al. Gene expression profiling in leiomyosarcomas and undifferentiated pleomorphic sarcomas: SRC as a new diagnostic marker. PLoS One 9:e102281 (2014).

2. Dou X & Charness ME Effect of lipid raft disruption on ethanol inhibition of l1 adhesion. Alcohol Clin Exp Res 38:2707-11 (2014).

Sequence Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Tissue Specificity

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Post-translational Modification

Myristoylated at Gly-2, and this is essential for targeting to membranes.; Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation. CDK5-mediated phosphorylation at Ser-75 targets SRC to ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. Phosphorylated by PTK2/FAK1; this enhances kinase activity. Phosphorylated by PTK2B/PYK2; this enhances kinase activity.; S-nitrosylation is important for activation of its kinase activity.; Ubiquitinated in response to CDK5-mediated phosphorylation. Ubiquitination mediated by CBLC requires SRC autophosphorylation at Tyr-419 and may lead to lysosomal degradation.

Subcellular Location

Cell membrane, Mitochondrion inner membrane, Cytoplasm, Nucleus.

UNIPROT

SwissProt: P12931 Human

SwissProt: P05480 Mouse

SwissProt: Q9WUD9 Rat

Synonyms

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

cSrc antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

OTTHUMP00000174476 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD Src cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD Src cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 54 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (10 µg/Lane)<br />Lane 2: A431 cell lysate (10 µg/Lane)<br />Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (20 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 6: Rat cerebellum tissue lysate (20 µg/Lane)<br />Lane 7: Rat lung tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (10 µg/Lane)
    Lane 2: A431 cell lysate (10 µg/Lane)
    Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)
    Lane 4: Mouse lung tissue lysate (20 µg/Lane)
    Lane 5: Mouse liver tissue lysate (20 µg/Lane)
    Lane 6: Rat cerebellum tissue lysate (20 µg/Lane)
    Lane 7: Rat lung tissue lysate (20 µg/Lane)

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A431 cells labeling Src with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of A431 cells labeling Src with Rabbit anti-Src antibody (ET1609-65) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Src antibody (ET1609-65) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-65) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-65) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Src antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-65) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of A431 cells labeling Src.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1609-65" style="font-weight: bold;text-decoration: underline;">ET1609-65</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling Src.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-65, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

SRC Rabbit Polyclonal Antibody

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-Src (Y419) Recombinant Rabbit Monoclonal Antibody [ST0800] - BSA and Azide free

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-Src (S17) Recombinant Rabbit Monoclonal Antibody [PSH11-03]

Application: WB,IF-Cell,IHC-P,FC,IP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

Src Recombinant Rabbit Monoclonal Antibody [JF0947] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

Phospho-Src (S17) Recombinant Rabbit Monoclonal Antibody [PSH11-03] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC,IP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

Phospho-Src (Y419) Recombinant Rabbit Monoclonal Antibody [ST0800]

Application: WB,FC,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Src Recombinant Rabbit Monoclonal Antibody [JF0947]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated