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TAG1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500013

TAG1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TAG1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human TAG1 aa 40-230.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 113 kDa

Positive Control

Mouse cerebellum tissue lysate, human brain tissue lysate, rat cerebellum tissue lysate, F9.

Conjugation

unconjugated

RRID

AB_3071113

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

Target

Function

This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy.

Background References

1. Thorpe HJ et al. Identification of CNTN2 as a genetic modifier of PIGA-CDG in a family with incomplete penetrance and in Drosophila. Am J Hum Genet. 2025 Mar

2. Lin ZJ et al. Gene-gene interaction network analysis indicates CNTN2 is a candidate gene for idiopathic generalized epilepsy. Neurogenetics. 2024 Apr

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q02246 Human

SwissProt: Q61330 Mouse

SwissProt: P22063 Rat

Synonyms

Axonal glycoprotein TAG 1 antibody

Axonal glycoprotein TAG-1 antibody

Axonal glycoprotein TAG1 antibody

Axonin 1 antibody

Axonin-1 antibody

Axonin1 antibody

AXT antibody

CNTN 2 antibody

Cntn2 antibody

CNTN2_HUMAN antibody

Expand

Images

  • Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500013" style="font-weight: bold;text-decoration: underline;">HA500013</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse cerebellum tissue lysate<br />Lane 2: Human brain tissue lysate<br />Lane 3: Rat cerebellum tissue lysate

    Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500013, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse cerebellum tissue lysate
    Lane 2: Human brain tissue lysate
    Lane 3: Rat cerebellum tissue lysate

  • ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500013" style="font-weight: bold;text-decoration: underline;">HA500013</a>, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500013, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation