TAGLN Recombinant Rabbit Monoclonal Antibody
Catalog# IRS202RB
TAGLN Recombinant Rabbit Monoclonal Antibody
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mIHC
-
Human
-
unconjugated
Overview
Product Name
TAGLN Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TAGLN aa 126-175 / 201.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Human skin tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization.
Background References
1. Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics 96:253-262 (2014).
2. Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M., Kornhauser J., Comb M.J. Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol. Cell. Proteomics 13:372-387 (2014).
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
22 kDa actin-binding protein antibody
Protein WS3-10 antibody
SM22 antibody
SM22-alpha antibody
Smooth muscle protein 22-alpha antibody
TAGL_HUMAN antibody
TAGLN antibody
Transgelin antibody
WS3-10 antibody
Images
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mIHC analysis of human skin tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-TAGLN antibody (IRS202RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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