TATA binding protein TBP Rabbit Polyclonal Antibody
Catalog# HA500527
TATA binding protein TBP Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TATA binding protein TBP Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human TBP aa 140-339 / 339.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, HepG2 cell lysate, MCF7 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, human bladder carcinoma tissue, human testis tissue.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:2,000
Target
Function
The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. This DNA sequence is found about 30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP is a member of a small gene family of TBP-related factors. The first TBP-related factor (TRF/TRF1) was identified in the fruit fly Drosophila, but appears to be fly or insect-specific. Subsequently TBPL1/TRF2 was found in the genomes of many metazoans, whereas vertebrate genomes encode a third vertebrate family member, TBPL2/TRF3. In specific cell types or on specific promoters TBP can be replaced by one of these TBP-related factors, some of which interact with the TATA box similarly to TBP.
Background References
1. Wang H et al. Structures and implications of TBP-nucleosome complexes. Proc Natl Acad Sci U S A. 2021 Jul
2. Magri S et al. Digenic inheritance of STUB1 variants and TBP polyglutamine expansions explains the incomplete penetrance of SCA17 and SCA48. Genet Med. 2022 Jan
Subcellular Location
Nucleus.
Synonyms
GTF2D antibody
GTF2D1 antibody
HDL4 antibody
MGC117320 antibody
MGC126054 antibody
MGC126055 antibody
SCA17 antibody
TATA binding factor antibody
TATA box factor antibody
TATA sequence binding protein antibody
ExpandImages
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Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: HCT 116 cell lysate (10 µg/Lane)
Lane 3: 293T cell lysate (10 µg/Lane)
Lane 4: HepG2 cell lysate (10 µg/Lane)
Lane 5: MCF7 cell lysate (10 µg/Lane)
Lane 6: Jurkat cell lysate (10 µg/Lane)
Lane 7: C2C12 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: PC-12 cell lysate (10 µg/Lane)
Lane 10: RAW264.7 cell lysate (10 µg/Lane)
Lane 11: Mouse testis tissue lysate (20 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 40/36 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500527) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
TATA binding protein TBP Recombinant Rabbit Monoclonal Antibody [PSH22-13]
Application: WB,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Products with the same target and pathway
TATA binding protein TBP Recombinant Rabbit Monoclonal Antibody [PSH22-13]
Application: WB,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
TATA binding protein TBP Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
