TDP43 Recombinant Rabbit Monoclonal Antibody [PSH09-14]
Catalog# HA723072
TDP43 Recombinant Rabbit Monoclonal Antibody [PSH09-14]
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WB
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IF-Cell
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IHC-P
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IHC-Fr
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IF-Tissue
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FC
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IP
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Human
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Mouse
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Rat
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HA751268
不含抗保成分
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Zebrafish
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
TDP43 Recombinant Rabbit Monoclonal Antibody [PSH09-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human TDP43 aa 365-414.
Species Reactivity
Human, Mouse, Rat (Predicted: Zebrafish, Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P, IHC-Fr, IF-Tissue, FC, IP
Molecular Weight
Predicted band size: 45 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate, HeLa, NIH/3T3, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
PSH09-14
Reactivity Data
| WB | IF-Cell | IHC-P | IHC-Fr | IF-Tissue | FC | IP | |
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| Human |
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| Mouse |
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| Rat |
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| Zebrafish |
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| Cynomolgus Monkey |
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| Pig |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IF-Cell
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1:500
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IHC-P
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1:1,000
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
TDP-43 is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. TDP-43 has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recent work has characterized the transcriptome-wide binding sites revealing that thousands of RNAs are bound by TDP-43 in neurons. TDP-43 was originally identified as a transcriptional repressor that binds to chromosomally integrated trans-activation response element (TAR) DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene and the apoA-II gene. In spinal motor neurons TDP-43 has also been shown in humans to be a low molecular weight neurofilament (hNFL) mRNA-binding protein. It has also shown to be a neuronal activity response factor in the dendrites of hippocampal neurons suggesting possible roles in regulating mRNA stability, transport and local translation in neurons. It has been demonstrated that zinc ions are able to induce aggregation of endogenous TDP-43 in cells. Moreover, zinc could bind to RNA binding domain of TDP-43 and induce the formation of amyloid-like aggregates in vitro.
Background References
1. McMillan M et al. RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia. Mol Cell. 2023 Jan
2. Corbet GA et al. TDP43 ribonucleoprotein granules: physiologic function to pathologic aggregates. RNA Biol. 2021 Oct
Subcellular Location
Cytoplasm, Mitochondrion, Nucleus.
Synonyms
ALS10 antibody
OTTHUMP00000002171 antibody
OTTHUMP00000002172 antibody
OTTHUMP00000002173 antibody
TADBP_HUMAN antibody
TAR DNA binding protein 43 antibody
TAR DNA binding protein antibody
TAR DNA-binding protein 43 antibody
TARDBP antibody
TDP 43 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Immunocytochemistry analysis of HeLa cells labeling TDP43 with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling TDP43 with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (HA723072) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of TDP43 on different lysates with Rabbit anti-TDP43 antibody (HA723072) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: Lane 1-3: 59 seconds; Lane 4-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723072) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TDP43 antibody (HA723072) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of NIH/3T3 cells labeling TDP43.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723072, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling TDP43.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723072, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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