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TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751089

TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TIE2 aa 1-769.

Species Reactivity

Human

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 126 kDa

Positive Control

EA.hy926 cell lysate, EA.hy926.

Conjugation

unconjugated

Clone Number

PSH06-63

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

Angiopoietin-1 receptor also known as CD202B (cluster of differentiation 202B) is a protein that in humans is encoded by the TEK gene. Also known as TIE2, it is an angiopoietin receptor. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. (TEK is closely related to the TIE receptor tyrosine kinase). This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. TEK has also been suggested as a marker for nucleus pulposus progenitor cells, from the intervertebral disc, which upon activation by Angiopoietin-1 starts to multiply and differentiate. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. In cancer patients, TEK (Tie2) is expressed in a subpopulation of monocytes that home in on the tumor and are essential for the formation of new blood vessels there.

Background References

1. Du S et al. Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancer. Cancer Lett. 2022 Mar

2. Duran CL et al. Targeting Tie2 in the Tumor Microenvironment: From Angiogenesis to Dissemination. Cancers (Basel). 2021 Nov

Subcellular Location

Cell membrane, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Secreted.

UNIPROT

SwissProt: Q02763 Human

Synonyms

Angiopoietin 1 receptor antibody

Angiopoietin-1 receptor antibody

CD202b antibody

CD202b antigen antibody

Endothelial tyrosine kinase antibody

Endothelium specific receptor tyrosine kinase 2 antibody

hTIE 2 antibody

hTIE2 antibody

Hyk antibody

p140 TEK antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TIE2 on different lysates with Rabbit anti-TIE2 antibody (<a href="/products/HA751089" style="font-weight: bold;text-decoration: underline;">HA751089</a>) at 1/1,000 dilution.<br /><br />Lane 1: EA.hy926 cell lysate<br />Lane 2: HEK-293 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 126 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751089" style="font-weight: bold;text-decoration: underline;">HA751089</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TIE2 on different lysates with Rabbit anti-TIE2 antibody (HA751089) at 1/1,000 dilution.

    Lane 1: EA.hy926 cell lysate
    Lane 2: HEK-293 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 126 kDa
    Observed band size: 150 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751089) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Flow cytometric analysis of HEK-293 (left, negative) and EA.hy926 (right, positive) cells labeling TIE2.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA751089" style="font-weight: bold;text-decoration: underline;">HA751089</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Relative expression (RE)

    Flow cytometric analysis of HEK-293 (left, negative) and EA.hy926 (right, positive) cells labeling TIE2.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751089, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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