TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH16-50]
Catalog# HA723854
TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH16-50]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH16-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
RPMI 8226 cell lysate, RAW264.7 cell lysates, human lung cancer tissue, mouse spleen tissue, rat spleen tissue.
Conjugation
unconjugated
Clone Number
PSH16-50
Reactivity Data
| WB | IHC-P | IP | |
|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
Target
Function
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 (TIM-3)gene. HAVCR2 was first described in 2002 as a cell surface molecule expressed on IFNγ producing CD4+ Th1 and CD8+ Tc1 cells. Later, the expression was detected in Th17 cells, regulatory T-cells, and innate immune cells (dendritic cells, NK cells, monocytes, macrophages). HAVCR2 receptor is a regulator of the immune response.
Background References
1. Kandel S et al. The TIM3/Gal9 signaling pathway: An emerging target for cancer immunotherapy. Cancer Lett. 2021 Jul
2. Palacios LM et al. TIM3 Expression in Anaplastic-Thyroid-Cancer-Infiltrating Macrophages: An Emerging Immunotherapeutic Target. Biology (Basel). 2022 Nov
Subcellular Location
Membrane, Cell junction, Cell membrane.
Synonyms
CD366 antibody
FLJ14428 antibody
HAVcr-2 antibody
Havcr2 antibody
HAVR2_HUMAN antibody
Hepatitis A virus cellular receptor 2 antibody
Kidney injury molecule 3 antibody
KIM 3 antibody
KIM3 antibody
T cell immunoglobulin and mucin domain containing 3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA723854) at 1/2,000 dilution.
Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (negative) (20 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723854) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TIM 3 on RAW264.7 cell lysates with Rabbit anti-TIM 3 antibody (HA723854) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 70 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723854) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TIM 3 antibody (HA723854) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723854) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TIM 3 antibody (HA723854) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723854) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TIM 3 antibody (HA723854) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723854) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
TIM 3 was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with HA723854 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723854 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RPMI 8226 cell lysate (input)
Lane 2: HA723854 IP in RPMI 8226 cell lysate
Lane 3: Rabbit IgG instead of HA723854 in RPMI 8226 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 seconds; ECL: K1801 -
TIM 3 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723854 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723854 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate (input)
Lane 2: HA723854 IP in RAW264.7 cell lysate
Lane 3: Rabbit IgG instead of HA723854 in RAW264.7 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 46 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"