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TIMP-1 Mouse Monoclonal Antibody [4C2]

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Catalog# EM2001-03

TIMP-1 Mouse Monoclonal Antibody [4C2]

Application

  • FC

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TIMP-1 Mouse Monoclonal Antibody [4C2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant human TIMP-1

Species Reactivity

Human

Validated Applications

FC, IF-Cell

Molecular Weight

21 kDa

Conjugation

unconjugated

Clone Number

4C2

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein G affinity purified.

Application Dilution

  • FC

  • 1ug/Test

  • IF-Cell

  • 1:50-1:200

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

Background References

暂无

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01033 Human

Synonyms

Clgi antibody

Collagenase inhibitor antibody

Collagenase inhibitor

Human antibody

EPA antibody

EPO antibody

Erythroid Potentiating Activity antibody

Erythroid-potentiating activity antibody

Fibroblast collagenase inhibitor antibody

FLJ90373 antibody

Expand

Images

  • The Hela (treated with 500nM BFA for 2 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-TIMP1 antibody (<a href="/products/EM2001-03" style="font-weight: bold;text-decoration: underline;">EM2001-03</a>): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

    The Hela (treated with 500nM BFA for 2 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-TIMP1 antibody (EM2001-03): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

  • 4% Paraformaldehyde-fixed Hela (treated with 500nM BFA for 2 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TIMP1) monoclonal Antibody, unconjugated (<a href="/products/EM2001-03" style="font-weight: bold;text-decoration: underline;">EM2001-03</a>) 1:100, 90 min at 37℃; followed by conjugated Goat Anti-Mouse IgG antibody (green, FITC) at 37℃ for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

    4% Paraformaldehyde-fixed Hela (treated with 500nM BFA for 2 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TIMP1) monoclonal Antibody, unconjugated (EM2001-03) 1:100, 90 min at 37℃; followed by conjugated Goat Anti-Mouse IgG antibody (green, FITC) at 37℃ for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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