TLR9 Mouse Monoclonal Antibody [H2-B12]
Catalog# EM1707-59
TLR9 Mouse Monoclonal Antibody [H2-B12]
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
TLR9 Mouse Monoclonal Antibody [H2-B12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. Coli.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC
Molecular Weight
116 kDa
Positive Control
Human TLR9 recombinant protein, TLR9-hIgGFc transfected HEK293 cell lysate, SK-OV-3, A549, human ?ovarian cancer tissue, human endometrial cancer tissue.
Conjugation
unconjugated
Clone Number
H2-B12
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:200-1:1,000
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IHC-P
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1:200-1:1,000
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FC
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1:200-1:400
Target
Function
The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NFκB signaling pathway, suggesting that they play a role in the immune response.
Background References
1. Chen GY et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012).
2. Chen GY et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009).
Subcellular Location
Endoplasmic reticulum membrane. Cell membrane
UNIPROT
Synonyms
CD 289 antibody
CD289 antibody
TLR 9 antibody
TLR9 antibody
TLR9_HUMAN antibody
Toll like receptor 9 antibody
Toll like receptor 9 isoform A precursor antibody
Toll like receptor 9 isoform B antibody
Toll-like receptor 9 antibody
Images
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Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using TLR9 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using TLR9 mouse mAb with DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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A novel mouse model of myositis-associated interstitial lung disease was established by using TLR9 agonist combined with muscle homogenate
Journal: Clinical And Experimental Immunology
DOI:
IF: 3.4
Application: IHC-P,WB
Reactivity: Mouse
Publish date: 2024 Nov
Alternative Products
TLR9 Recombinant Rabbit Monoclonal Antibody [JE59-91]
Application: WB
Reactivity: Human
Conjugate: unconjugated
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