TMED9 Recombinant Mouse Monoclonal Antibody [A9A7-R]
Catalog# HA601129
TMED9 Recombinant Mouse Monoclonal Antibody [A9A7-R]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TMED9 Recombinant Mouse Monoclonal Antibody [A9A7-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human TMED9 aa 31-220 / 235.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 27 kDa
Positive Control
Hela cell lysate, HepG2 cell lysate, LO2 cell lysate, THP-1 cell lysate, A431 cell lysate, A549 cell lysate, MCF-7 cell lysate, Raji cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse liver tissue lysate, human liver tissue, human lung tissue, human pancreas tissue, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue.
Conjugation
unconjugated
Clone Number
A9A7-R
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:200-1:1,000
Target
Function
Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB.
Background References
1. Li S et al. A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. Cell Res. 2022 Feb
2. Puri C et al. TMED9-SEC12, an important "contact" for autophagy. Cell Res. 2022 Feb
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus, Endoplasmic reticulum-Golgi intermediate compartment membrane.
Synonyms
Transmembrane emp24 domain-containing protein 9
GMP25
Glycoprotein 25L2
p24 family protein alpha-2 (p24alpha2)
p25
TMED9
GP25L2
Images
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Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LO2 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: A431 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: Raji cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: Human lung tissue lysate
Lane 12: Mouse lung tissue lysate
Lane 13: Mouse liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 10 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-TMED9 antibody (HA601129) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-TMED9 antibody (HA601129) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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