TMEM39a Rabbit Polyclonal Antibody
Catalog# R1510-16
TMEM39a Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
TMEM39a Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Human TMEM39a aa 475-488 / 488 Human.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 56 kDa
Positive Control
Hela, PANC-1, human pancreas tissue, human colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:600
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FC
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1:100
Target
Function
TMEM39A is a 146 amino acid protein encoded by a gene mapping to human chromosome 3. Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.
Background References
1. Ota T., Suzuki Y., Nishikawa T., et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Genet. 36:40-45(2004).
2. Sjoeblom T., Jones S., Wood L.D., et al. The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).
Sequence Similarity
Belongs to the TMEM39 family.
Subcellular Location
Endoplasmic reticulum membrane.
UNIPROT
Synonyms
Hypothetical protein FLJ10902 antibody
Transmembrane protein 39A antibody
Images
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Immunocytochemical staining of Hela cells using anti-TMEM39a rabbit polyclonal antibody.
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Immunocytochemical staining of PANC-1 cells using anti-TMEM39a rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-TMEM39a rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TMEM39a antibody (R1510-16) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-16) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"