TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30] - BSA and Azide free
Catalog# HA750708
TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TRAF3 301-568 / 568.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 64 kDa
Positive Control
TF-1 cell lysate, THP-1 cell lysate, 293T cell lysate, Jurkat cell lysate, A549 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH01-30
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
TNF receptor-associated factor (TRAF3) is a protein that in humans is encoded by the TRAF3 gene. The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus-encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Background References
1. Gao P et al. E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3. Nat Commun. 2021 Feb
2. Sun N et al. TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2. Protein Cell. 2020 Dec
Subcellular Location
Cytoplasm, Endosome, Mitochondrion.
Synonyms
CAP 1 antibody
CAP-1 antibody
CAP1 antibody
CD40 associated protein 1 antibody
CD40 binding protein antibody
CD40 bp antibody
CD40 receptor associated factor 1 antibody
CD40 receptor-associated factor 1 antibody
CD40-binding protein antibody
CD40BP antibody
ExpandImages
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This data was developed using HA721654, the same antibody clone in a different buffer formulation.
Western blot analysis of TRAF3 on different lysates with Rabbit anti-TRAF3 antibody (HA721654) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Rat spleen tissue lysate (40 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 50 kDa
Exposure time: 1 minute 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721654) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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