TROP2 Rabbit Polyclonal Antibody
Catalog# ER1802-60
TROP2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
TROP2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within RFc-hTROP2 aa 27-274 (Extracellular)/323.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC, IF-Tissue
Molecular Weight
Predicted band size: 36 kDa
Positive Control
A431 cell lysates, A431, human breast carcinoma tissue, human lung carcinoma tissue, human liver carcinoma tissue, human colon carcinoma tissue, human esophagus tissue, Hela, human breast carcinoma tissue, human skin tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:5,000
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FC
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1:50-1:100
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IF-Tissue
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1:50-1:100
Target
Function
TROP-2, also known as tumor-associated calcium signal transducer 2 (TACSTD2), pancreatic carcinoma marker protein GA733-1, membrane component chromosome 1, surface marker 1 (M1S1) or epithelial glycoprotein-1 (EGP-1), is a cell surface glycoprotein receptor. It is a single pass type I membrane protein containing one thryoglobulin type-1 domain, an epidermal growth factor-like repeat, a phosphatidylinositol binding site and tyrosine phosphorylation sites near the C-terminus. TROP-2 plays a role in tranducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multistratified epithelia. It is also highly expressed in several types of tumors and is involved in regulating the growth of carcinoma cells. Mutations in the gene encoding TROP-2 can result in gelatinous drop-like corneal dystrophy (GDLD) also referred to as lattice corneal dystrophy type III, an autosomal recessive disorder that causes severe visual impairment.
Background References
1. TROP2 methylation and expression in tamoxifen-resistant breast cancer. Zimmers SM. et. al. Cancer Cell Int. 2018 Jul
2. High expression of TROP2 is correlated with poor prognosis of oral squamous cell carcinoma. Tang G. et. al. Pathol Res Pract. 2018 Oct
Sequence Similarity
Belongs to the EPCAM family.
Tissue Specificity
Placenta, pancreatic carcinoma cell lines.
Post-translational Modification
The N-terminus is blocked.
Subcellular Location
Membrane.
UNIPROT
Synonyms
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-2 antibody
Cell surface glycoprotein Trop2 antibody
Epithelial glycoprotein 1 antibody
GA733 1 antibody
GA7331 antibody
M1S 1 antibody
M1S1 antibody
Membrane component chromosome 1 surface marker 1 antibody
Pancreatic carcinoma marker protein GA733 1 antibody
ExpandImages
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Western blot analysis of TROP2 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1802-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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ICC staining of TROP2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1802-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TROP2 antibody (ER1802-60) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-60) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-TROP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TROP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-TROP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-TROP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of TROP2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1802-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
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Immunofluorescence analysis of paraffin-embedded human breast carcinoma tissue labeling TROP2 with Rabbit anti-TROP2 antibody (ER1802-60) at 1/85 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-60, red) at 1/85 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling TROP2 with Rabbit anti-TROP2 antibody (ER1802-60) at 1/85 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-60, red) at 1/85 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"