TRPV4 Recombinant Rabbit Monoclonal Antibody [PSH16-51]
Catalog# HA723855
TRPV4 Recombinant Rabbit Monoclonal Antibody [PSH16-51]
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WB
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IHC-Fr
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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HA751600
不含抗保成分
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unconjugated
Overview
Product Name
TRPV4 Recombinant Rabbit Monoclonal Antibody [PSH16-51]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-Fr, IHC-P, IF-Tissue, IP
Molecular Weight
Predicted band size: 98 kDa
Positive Control
A375 cell lysate, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
PSH16-51
Reactivity Data
| WB | IHC-Fr | IHC-P | IF-Tissue | IP | |
|---|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-Fr
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1:500
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IHC-P
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1:1,000
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IF-Tissue
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1:500
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IP
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1-2μg/sample
Target
Function
Transient receptor potential cation channel subfamily V member 4 is an ion channel protein that in humans is encoded by the TRPV4 gene. The TRPV4 gene encodes TRPV4, initially named "vanilloid-receptor related osmotically activated channel" (VR-OAC) and "OSM9-like transient receptor potential channel, member 4 (OTRPC4)", a member of the vanilloid subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that has been found involved in multiple physiologic functions, dysfunctions and also disease. It functions in the regulation of systemic osmotic pressure by the brain, in vascular function, in liver, intestinal, renal and bladder function, in skin barrier function and response of the skin to ultraviolet-B radiation, in growth and structural integrity of the skeleton, in function of joints, in airway- and lung function, in retinal and inner ear function, and in pain. The channel is activated by osmotic, mechanical and chemical cues. It also responds to thermal changes (warmth). Channel activation can be sensitized by inflammation and injury.
Background References
1. Huang T et al. Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. Autophagy. 2021 Nov
2. Lan Z et al. Mechanosensitive TRPV4 is required for crystal-induced inflammation. Ann Rheum Dis. 2021 Dec
Subcellular Location
Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium.
Synonyms
BCYM3 antibody
CMT2C antibody
HMSN2C antibody
osm 9 like TRP channel 4 antibody
Osm-9-like TRP channel 4 antibody
OSM9 like transient receptor potential channel 4 antibody
Osmosensitive transient receptor potential channel 4 antibody
OTRPC 4 antibody
OTRPC4 antibody
SMAL antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of TRPV4 on different lysates with Rabbit anti-TRPV4 antibody (HA723855) at 1/1,000 dilution.
Lane 1: A375 cell lysate
Lane 2: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 90 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723855) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: kidney
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TRPV4 antibody (HA723855) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723855) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
TRPV4 was immunoprecipitated from 0.2 mg A375 cell lysate with HA723855 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723855 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A375 cell lysate (input)
Lane 2: HA723855 IP in A375 cell lysate
Lane 3: Rabbit IgG instead of HA723855 in A375 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 59 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TRPV4 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
