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TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]

Usd: 385

Specification

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Catalog# HA721211

TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TdT aa 1-509.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 59 kDa

Positive Control

Jurkat cell lysate, MOLT-4 cell lysate, human thymus tissue.

Conjugation

unconjugated

Clone Number

PD01-00

RRID

AB_3072331

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that changes the addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator without template direction. TdT contributes to the generation of junctional diversity in antigen receptors of immature lymphocytes. TdT is expressed in lymphoid precursors of B- and T-cell lineage in thymus and bone marrow. Foci of TdT positive cells maybe observed in peripheral lymphoid tissues. TdT is also present in malignant tumors of lymphoblastic lineage and thymoma. It is a sensitive and specific marker for lymphoblastic lymphoma/leukemia. Anti-TdT is a useful marker for lymphoblastic lymphomas and has been observed in some cases of acute myeloid leukemia.

Background References

1. Musallam KM et al. Revisiting the non-transfusion-dependent (NTDT) vs. transfusion-dependent (TDT) thalassemia classification 10 years later. Am J Hematol. 2021 Feb

2. Ali M et al. T cells targeted to TdT kill leukemic lymphoblasts while sparing normal lymphocytes. Nat Biotechnol. 2022 Apr

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P04053 Human

Synonyms

Deoxynucleotidyltransferase terminal antibody

DNA nucleotidylexotransferase antibody

DNTT antibody

Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody

TDT antibody

TDT_HUMAN antibody

Terminal addition enzyme antibody

Terminal deoxynucleotidyltransferase antibody

Terminal deoxyribonucleotidyltransferase antibody

Terminal transferase antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Raji cell lysate (negative) (20 µg/Lane)<br />Lane 4: Daudi cell lysate (negative) (20 µg/Lane)<br />Lane 5: MOLT-4 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (HA721211) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (negative) (20 µg/Lane)
    Lane 3: Raji cell lysate (negative) (20 µg/Lane)
    Lane 4: Daudi cell lysate (negative) (20 µg/Lane)
    Lane 5: MOLT-4 cell lysate (20 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TdT antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TdT antibody (HA721211) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721211) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TdT antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721211" style="font-weight: bold;text-decoration: underline;">HA721211</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TdT antibody (HA721211) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721211) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"