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UAP1 Recombinant Mouse Monoclonal Antibody [4H1-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA601660

UAP1 Recombinant Mouse Monoclonal Antibody [4H1-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

UAP1 Recombinant Mouse Monoclonal Antibody [4H1-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human UAP1 aa 33-82 / 522.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 59 kDa

Positive Control

SK-Br-3 cell lysate, Jurkat cell lysate, NCI-H1299 cell lysate.

Conjugation

unconjugated

Clone Number

4H1-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Background References

1. Wang-Gillam A et al. A 17-amino acid insert changes UDP-N-acetylhexosamine pyrophosphorylase specificity from UDP-GalNAc to UDP-GlcNAc. J Biol Chem 273:27055-27057 (1998).

2. Peneff C et al. Crystal structures of two human pyrophosphorylase isoforms in complexes with UDPGlc(Gal)NAc: role of the alternatively spliced insert in the enzyme oligomeric assembly and active site architecture. EMBO J 20:6191-6202 (2001).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q16222 Human

Synonyms

AGX 1 antibody

AGX antibody

AGX-1 antibody

AGX-2 antibody

AGX1 antibody

Antigen X antibody

AntigenX antibody

SPAG 2 antibody

SPAG2 antibody

Sperm associated antigen 2 antibody

Expand

Images

  • Western blot analysis of UAP1 on different lysates with Mouse anti-UAP1 antibody (<a href="/products/HA601660" style="font-weight: bold;text-decoration: underline;">HA601660</a>) at 1/4,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: NCI-H1299 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 57/59 kDa<br /><br />Exposure time: 1 minute 14 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601660" style="font-weight: bold;text-decoration: underline;">HA601660</a>) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of UAP1 on different lysates with Mouse anti-UAP1 antibody (HA601660) at 1/4,000 dilution.

    Lane 1: SK-Br-3 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: NCI-H1299 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 57/59 kDa

    Exposure time: 1 minute 14 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601660) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"