UBE1C Rabbit Polyclonal Antibody
Catalog# R1706-14
UBE1C Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
UBE1C Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human UBE1C aa 220-424 / 463.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Human liver tissue lysate, A431, HUVEC, SH-SY5Y, rat brain tissue, human pancreas tissue, mouse testis tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation. UBA3 (ubiquitin-like modifier activating enzyme 3), also known as NEDD8-activating enzyme E1 catalytic subunit or UBE1C (ubiquitin-activating enzyme E1C), is a 463 amino acid protein belonging to the ubiquitin-activating E1 family and UBA3 subfamily. Ubiquitously expressed, UBA3 acts as an activator to NEDD8, a ubiquitin-like protein, thus regulating cell division, signaling and embryogenesis. UBA3 exists as two isoforms due to alternative splicing events.
Background References
1. Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017).
2. Xu J et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015).
Sequence Similarity
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
Tissue Specificity
Ubiquitously expressed.
Subcellular Location
Nucleus. Cytoplasm.
Synonyms
DKFZp566J164 antibody
EC 6.3.2. antibody
hUba3 antibody
MGC22384 antibody
NEDD8 activating enzyme E1 catalytic subunit antibody
NEDD8 activating enzyme E1C antibody
Nedd8 activating enzyme hUba3 antibody
NEDD8-activating enzyme E1 catalytic subunit antibody
NEDD8-activating enzyme E1C antibody
uba3 antibody
ExpandImages
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Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.
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ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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