USP21 Rabbit Polyclonal Antibody
Catalog# ER1802-25
USP21 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
USP21 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human USP21 aa 1-50 / 565.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
63 kDa
Positive Control
A549 cell lysate, HepG2 cell lysate, PC-3M, SH-SY-5Y, human liver cancer tissue, human kidney tissue, human fetal skeletal muscle tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5000
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IF-Cell
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.
Background References
1. Gong L et al. Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins. J Biol Chem 275:14212-14216 (2000).
2. Khan A et al. BEND3 represses rDNA transcription by stabilizing a NoRC component via USP21 deubiquitinase. Proc Natl Acad Sci USA 112:8338-8343 (2015).
Sequence Similarity
Belongs to the peptidase C19 family. USP21 subfamily.
Tissue Specificity
Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
Deubiquitinating enzyme 21 antibody
deubiquitinating enzyme 23 antibody
EC 3.1.2.15 antibody
ESTM28 antibody
MGC3394 antibody
NEDD8-specific protease antibody
Ubiquitin carboxyl terminal hydrolase 21 antibody
ubiquitin carboxyl terminal hydrolase 23 antibody
Ubiquitin carboxyl-terminal hydrolase 21 antibody
ubiquitin specific peptidase 21 antibody
ExpandImages
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Western blot analysis of USP21 on different lysates with Rabbit anti-USP21 antibody (ER1802-25) at 1/5,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 180 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-25, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 59 kDa -
ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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