USP28 Mouse Monoclonal Antibody [A1F9]
Catalog# EM1901-46
USP28 Mouse Monoclonal Antibody [A1F9]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
USP28 Mouse Monoclonal Antibody [A1F9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human USP28 aa 509-860 / 1,077.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 122 kDa
Positive Control
HeLa cell lysate, A431 cell lysate, 293T cell lysate, SW480 cell lysate, LNCaP cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
A1F9
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
Background References
1. Serra R.W. et. al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 3:E02313-E02313(2014).
2. Bassermann F. et. al. The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint. Cell 134:256-267(2008).
Sequence Similarity
Belongs to the peptidase C19 family. USP28 subfamily.
Post-translational Modification
Degraded upon nickel ion level or hypoxia exposure.; Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.
Subcellular Location
Nucleoplasm.
Synonyms
Deubiquitinating enzyme 28 antibody
KIAA1515 antibody
Ubiquitin carboxyl terminal hydrolase 28 antibody
Ubiquitin carboxyl-terminal hydrolase 28 antibody
ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
Ubiquitin specific peptidase 28 antibody
Ubiquitin specific processing protease 28 antibody
Ubiquitin specific protease 28 antibody
Ubiquitin thioesterase 28 antibody
Ubiquitin thiolesterase 28 antibody
ExpandImages
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Western blot analysis of USP28 on different lysates with Mouse anti-USP28 antibody (EM1901-46) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: 293T cell lysate
Lane 4: SW480 cell lysate
Lane 5: LNCaP cell lysate
Lane 6: MDA-MB-231 cell lysate
Lane 7: MDA-MB-468 cell lysate
Lane 8: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 122 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
USP28 Mouse Monoclonal Antibody [A1F8]
Application: WB
Reactivity: Human
Conjugate: unconjugated
