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UTF1 Mouse Monoclonal Antibody [E0-F4]

Usd: 350

Specification

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Catalog# M1407-6

UTF1 Mouse Monoclonal Antibody [E0-F4]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

UTF1 Mouse Monoclonal Antibody [E0-F4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human UTF1 aa 51-100 / 341.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

36-50 kDa

Positive Control

D3

Conjugation

unconjugated

Clone Number

E0-F4

RRID

AB_3073087

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:500-1:1,000

Target

Function

Undifferentiated embryonic cell transcription factor 1 is a protein in humans that is encoded by the UTF1 gene. UTF1, first reported in 1998, is expressed in pluripotent cells including embryonic stem cells and embryonic carcinoma cells. Its expression is rapidly reduced upon differentiation. UTF1 protein is localized to the cell nucleus, where it functions to regulate the pluripotent chromatin state and buffer mRNA levels by promoting degradation of mRNA. Aberrant expression of UTF1 has also been reported in cervical cancer cells, where the UTF1 gene promoter loses methylation and becomes abnormally expressed compared to normal cervical cells.

Background References

1. "Characterization of functional domains of an embryonic stem cell coactivator UTF1 which are conserved and essential for potentiation of ATF-2 activity." Fukushima A., Okuda A., Nishimoto M., Seki N., Hori T.A., Muramatsu M. J. Biol. Chem. 273:25840-25849(1998)

2. "Structural analyses of the UTF1 gene encoding a transcriptional coactivator expressed in pluripotent embryonic stem cells." Nishimoto M., Fukushima A., Miyagi S., Suzuki Y., Sugano S., Matsuda Y., Hori T., Muramatsu M., Okuda A. Biochem. Biophys. Res. Commun. 285:945-953(2001)

3. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011)

Post-translational Modification

Phosphorylated.

Subcellular Location

Nucleus

UNIPROT

SwissProt: Q5T230 Human

Synonyms

hUTF 1 antibody

hUTF1 antibody

Undifferentiated embryonic cell transcription factor 1 antibody

UTF 1 antibody

UTF1 antibody

UTF1_HUMAN antibody

Images

  • Western blot analysis on D3 cell lysates using anti- UTF1 mouse mAb.

    Western blot analysis on D3 cell lysates using anti- UTF1 mouse mAb.

  • ICC staining UTF1 in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining UTF1 in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation