VE-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH27-80]
Catalog# HA724725
VE-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH27-80]
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WB
-
IF-Tissue
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IHC-P
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IP
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Mouse
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Rat
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unconjugated
Overview
Product Name
VE-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH27-80]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse VE-Cadherin aa 1-599.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P, IP
Target Molecular Weight
Predicted band size: 88 kDa
Positive Control
BEnd.3 (Mouse brain microvascular endothelial cell) cell lysate, Mouse lung tissue lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate, Rat lung tissue lysate, Rat kidney tissue lysate, Rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH27-80
Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Tissue
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1:100
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IHC-P
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1:500
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IP
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1-2μg/sample
Target
Function
The cadherins are a family of Ca2+ dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.
Background References
1. Ye X et al. Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma. J Pathol 239:139-51 (2016).
2. Li C et al. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. Nat Commun 7:11455 (2016).
Subcellular Location
Cell junction, adherens junction, Cell membrane, Cytoplasm.
Synonyms
7B 4 antibody
7B4 antibody
7B4 antigen antibody
CADH5_HUMAN antibody
Cadherin 5 antibody
Cadherin 5 type 2 antibody
Cadherin 5, type 2 (vascular endothelium) antibody
Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
cadherin, vascular endothelial antibody
cadherin, vascular endothelial, 1 antibody
ExpandImages
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☑ Relative expression (RE),Independent antibody validation (IAV)
Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (HA724725) at 1/2,000 dilution.
Lane 1: bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate (15 µg/Lane)
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate (15 µg/Lane)
Exposure time: 25 seconds; ECL: K1801
Low expression of VE-Cadherin protein in NIH/3T3 cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724725, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 88 kDa
Observed band size: 90-150 kDa -
☑ Independent antibody validation (IAV)
Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (HA724725) at 1/2,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat lung tissue lysate
Lane 7: Rat kidney tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724725, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 88 kDa
Observed band size: 90-150 kDa -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724725, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724725, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Immunoprecipitation (IP)
VE-Cadherin was immunoprecipitated in 0.2 mg bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate with HA724725 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724725 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: bEnd.3 cell lysate (input)
Lane 2: HA724725 IP in bEnd.3 cell lysate
Lane 3: Rabbit IgG instead of HA724725 in bEnd.3 cell lysate
Exposure time: 2 minutes 6 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724725, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 88 kDa
Observed band size: 90-150 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"