WNT8A Recombinant Rabbit Monoclonal Antibody [PSH0-39]
Catalog# HA721341
WNT8A Recombinant Rabbit Monoclonal Antibody [PSH0-39]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
WNT8A Recombinant Rabbit Monoclonal Antibody [PSH0-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human WNT8A aa 100-300 / 351.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 38.8 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse embryo tissue lysate, rat brain tissue lysate, NIH/3T3, HeLa.
Conjugation
unconjugated
Clone Number
PSH0-39
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
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|
|
| Mouse |
|
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50
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FC
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1:500-1:1,000
Target
Function
Protein Wnt-8a is a protein that in humans is encoded by the WNT8A gene. Wnt8a may be involved in the development of early embryos as well as germ cell tumors. The Wnt8a gene is part of the Wnt family of genes and plays a role in vertebrates in the process of axis patterning. Wnt8a encodes for 2 proteins, Wnt8a.1 and Wnt8a.2 via a complicated mechanism involving the coordination of signaling molecules to control up and down stream promoters. Furthermore, Wnt8a has shown to play a role in neural crest induction via Wnt/β-catenin signaling based on experiments using zebrafish as a model organism. Wnt8a among other Wnt genes influence the Wnt/β-catenin signaling in neural crest development. β-catenin is degraded in the absence of a Wnt signaling, but upon the binding of a Wnt ligand with a frizzled receptor and Lrp5/6 the β-catenin signaling molecule is no longer degraded. Instead, it interacts with transcription factors that activate Wnt expression, of which, Wnt8 is crucial for neural crest development and other cell fates. In a study of morpholino based gene knockdown in zebrafish, knocking down Wnt8a resulted in the lack of expression of various other genes important for neural crest induction (pax3, sox10, and foxd3). It is unclear however, if the loss of these neural crest specifiers is a result of Wnt8a directly in the induction process or is a downstream consequence of disruptions in Wnt8a signaling earlier in development. Regardless, the results of this study show Wnt8a as a key player in neural crest induction.
Background References
1. Hino H et al. Roles of maternal wnt8a transcripts in axis formation in zebrafish. Dev Biol. 2018 Feb
2. Naylor RW et al. Wnt8a expands the pool of embryonic kidney progenitors in zebrafish. Dev Biol. 2017 May
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
Protein Wnt-8a antibody
Protein Wnt-8d antibody
wingless type MMTV integration site family, member 8A antibody
WNT 8A antibody
Wnt 8d antibody
wnt8a antibody
WNT8A_HUMAN antibody
WNT8D antibody
Images
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Western blot analysis of WNT8A on different lysates with Rabbit anti-WNT8A antibody (HA721341) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HepG2 cell lysate (30 µg/Lane)
Lane 3: SW480 cell lysate (30 µg/Lane)
Lane 4: Jurkat cell lysate (30 µg/Lane)
Lane 5: Raji cell lysate (30 µg/Lane)
Lane 6: NIH/3T3 cell lysate (30 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Mouse embryo tissue lysate (40 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 38.8 kDa
Observed band size: 55 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721341) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling WNT8A with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling WNT8A.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721341, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling WNT8A.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721341, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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