WTAP Mouse Monoclonal Antibody [B6-B6]
Catalog# EM1706-68
WTAP Mouse Monoclonal Antibody [B6-B6]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Monkey
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unconjugated
Overview
Product Name
WTAP Mouse Monoclonal Antibody [B6-B6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
Species Reactivity
Human, Monkey
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
55 kDa
Positive Control
Human WTAP recombinant protein, WTAP-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela, K562, Hek293, A549, HepG2, Jurkat, Cos7, human cervical cancer tissue, human endometrial cancer tissue.
Conjugation
unconjugated
Clone Number
B6-B6
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-cell
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1:200-1:1,000
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IHC-P
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1:200-1:1,000
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FC
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1:200-1:400
Target
Function
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.
Background References
1. Jo HJ et al. WTAP regulates migration and invasion of cholangiocarcinoma cells. J Gastroenterol 48(11):1271-82 (2013).
2. Jin DI et al. Expression and roles of Wilms\' tumor 1-associating protein in glioblastoma. Cancer Sci 103(12):2102-9 (2012).
Subcellular Location
Nucleus, nucleolus
UNIPROT
Synonyms
DKFZp686F20131 antibody
Female-lethal(2)D homolog antibody
FL2D_HUMAN antibody
hFL(2)D antibody
KIAA0105 antibody
MGC3925 antibody
Mum2 antibody
OTTHUMP00000017522 antibody
OTTHUMP00000017523 antibody
PNAS 132 antibody
ExpandImages
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Western blot analysis of WTAP on human WTAP recombinant protein using anti-WTAP antibody at 1/1,000 dilution.
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Western blot analysis of WTAP on HEK293 (1) and WTAP-hIgGFc transfected HEK293 (2) cell lysate using anti-WTAP antibody at 1/1,000 dilution.
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Western blot analysis of WTAP on different cell lysate using anti-WTAP antibody at 1/1,000 dilution.
Positive control: Line1: MCF-7 Line2: Hela Line3: K562 Line4: Hek293 Line5: A549 Line6: HepG2 Line7: Jurkat Line8: Cos7 -
ICC staining WTAP (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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