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XIAP Recombinant Rabbit Monoclonal Antibody [PSH04-30]

Usd: 385

Specification

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Catalog# HA722113

XIAP Recombinant Rabbit Monoclonal Antibody [PSH04-30]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

XIAP Recombinant Rabbit Monoclonal Antibody [PSH04-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human XIAP aa 301-450 / 497.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

293T cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human breast cancer tissue, human colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

PSH04-30

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50

Target

Function

X-linked inhibitor of apoptosis protein (XIAP), also known as inhibitor of apoptosis protein 3 (IAP3) and baculoviral IAP repeat-containing protein 4 (BIRC4), is a protein that stops apoptotic cell death. In humans, this protein (XIAP) is produced by a gene named XIAP gene located on the X chromosome. XIAP is a member of the inhibitor of apoptosis family of proteins (IAP). IAPs were initially identified in baculoviruses, but XIAP is one of the homologous proteins found in mammals. It is so called because it was first discovered by a 273 base pair site on the X chromosome. The protein is also called human IAP-like Protein (hILP), because it is not as well conserved as the human IAPS: hIAP-1 and hIAP-2. XIAP is the most potent human IAP protein currently identified.

Background References

1. Hanifeh M et al. XIAP as a multifaceted molecule in Cellular Signaling. Apoptosis. 2022 Aug

2. Tu H et al. XIAP\'s Profile in Human Cancer. Biomolecules. 2020 Oct

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P98170 Human

SwissProt: Q60989 Mouse

SwissProt: Q9R0I6 Rat

Synonyms

AP 13 antibody

API3 antibody

Apoptosis Inhibitor 3 antibody

Baculoviral IAP repeat containing 4 antibody

Baculoviral IAP Repeat Containing Protein 4 antibody

Baculoviral IAP repeat-containing protein 4 antibody

BIRC 4 antibody

BIRC4 antibody

E3 ubiquitin-protein ligase XIAP antibody

hIAP-3 antibody

Expand

Images

  • Western blot analysis of XIAP on different lysates with Rabbit anti-XIAP antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: A549 cell lysate (20 µg/Lane)<br />Lane 5: HeLa cell lysate (20 µg/Lane)<br />Lane 6: COS-1 cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 10: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 57 kDa<br />Observed band size: 50 kDa<br />Exposure time: Lane 1-10 (left): 20 seconds; Lane 1-10 (right): 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of XIAP on different lysates with Rabbit anti-XIAP antibody (HA722113) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: A549 cell lysate (20 µg/Lane)
    Lane 5: HeLa cell lysate (20 µg/Lane)
    Lane 6: COS-1 cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: Mouse brain tissue lysate (20 µg/Lane)
    Lane 10: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 57 kDa
    Observed band size: 50 kDa
    Exposure time: Lane 1-10 (left): 20 seconds; Lane 1-10 (right): 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722113) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of XIAP with Rabbit anti-XIAP antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/2,000 dilution.<br /><br />Lane 1: Wild-type Caco-2 whole cell lysate (10 µg/Lane)<br />Lane 2/3: XIAP knockdown Caco-2 whole cell lysate (10 µg/Lane)<br /><br />Predicted band size: 57 kDa<br />Observed band size: 50 kDa<br />Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel.<br /><br /><a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a> was shown to specifically react with XIAP in wild-type Caco-2 cells. Weakened bands were observed when XIAP knockdown samples were tested. Wild-type and XIAP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of XIAP with Rabbit anti-XIAP antibody (HA722113) at 1/2,000 dilution.

    Lane 1: Wild-type Caco-2 whole cell lysate (10 µg/Lane)
    Lane 2/3: XIAP knockdown Caco-2 whole cell lysate (10 µg/Lane)

    Predicted band size: 57 kDa
    Observed band size: 50 kDa
    Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel.

    HA722113 was shown to specifically react with XIAP in wild-type Caco-2 cells. Weakened bands were observed when XIAP knockdown samples were tested. Wild-type and XIAP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA722113) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-XIAP antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-XIAP antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-XIAP antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722113" style="font-weight: bold;text-decoration: underline;">HA722113</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

XIAP Recombinant Rabbit Monoclonal Antibody [PSH04-30] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated