Xanthine Oxidase Recombinant Rabbit Monoclonal Antibody [JG38-40]
Catalog# ET7108-69
Xanthine Oxidase Recombinant Rabbit Monoclonal Antibody [JG38-40]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Xanthine Oxidase Recombinant Rabbit Monoclonal Antibody [JG38-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Xanthine Oxidase aa 182-313 / 1333.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 146 kDa
Positive Control
Human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
JG38-40
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid. Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid. The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification. Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues. In the oxidase form, this enzyme is coupled to the generation of free radicals. Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction. Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally. The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders.
Background References
1. Yamaguchi Y et al. Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate. J Biochem 141:513-524 (2007).
2. Saksela M et al. Cloning and expression in vitro of human xanthine dehydrogenase/oxidase. Biochem J 315:235-239 (1996).
Sequence Similarity
Belongs to the xanthine dehydrogenase family.
Tissue Specificity
Detected in milk (at protein level).
Post-translational Modification
Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).; Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Subcellular Location
Cytoplasm. Peroxisome. Secreted.
Synonyms
Xanthine dehydrogenase antibody
Xanthine dehydrogenase/oxidase antibody
Xanthine oxidase antibody
Xanthine oxidoreductase antibody
XD antibody
XDH antibody
XDH_HUMAN antibody
xdha antibody
XO antibody
xor antibody
Images
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Western blot analysis of Xanthine Oxidase on different lysates with Rabbit anti-Xanthine Oxidase antibody (ET7108-69) at 1/1,000 dilution.
Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 146 kDa
Observed band size: 146 kDa
Exposure time: Lane 1: 1 minute 2 seconds; Lane 2-3: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"