YKL-40 / CHI3L1 Recombinant Rabbit Monoclonal Antibody [PSH16-26]
Catalog# HA723824
YKL-40 / CHI3L1 Recombinant Rabbit Monoclonal Antibody [PSH16-26]
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WB
-
IF-Cell
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Human
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unconjugated
Overview
Product Name
YKL-40 / CHI3L1 Recombinant Rabbit Monoclonal Antibody [PSH16-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
THP-1 cell lysate, THP-1.
Conjugation
unconjugated
Clone Number
PSH16-26
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:50
Target
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Background References
1. Connolly K et al. Potential role of chitinase-3-like protein 1 (CHI3L1/YKL-40) in neurodegeneration and Alzheimer\'s disease. Alzheimers Dement. 2023 Jan
2. Yu JE et al. Significance of chitinase-3-like protein 1 in the pathogenesis of inflammatory diseases and cancer. Exp Mol Med. 2024 Feb
Subcellular Location
Secreted, extracellular space, Cytoplasm, perinuclear region, Endoplasmic reticulum.
UNIPROT
Synonyms
39 kDa synovial protein antibody
ASRT7 antibody
Cartilage glycoprotein 39 antibody
CGP-39 antibody
CGP39 antibody
CH3L1_HUMAN antibody
CHI3L1 antibody
chitinase 3 like 1 (cartilage glycoprotein 39) antibody
chitinase 3 like 1 antibody
Chitinase 3 like protein 1 precursor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of YKL-40 / CHI3L1 on different lysates with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 250 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723824) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of THP-1 (positive) and 293T (negative) labeling YKL-40 / CHI3L1 with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"