ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]
Catalog# HA721886
ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ZHX2 aa 251-500 / 837.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 92 kDa
Positive Control
Neuro-2a cell lysate, COS-1 cell lysate, HeLa cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, MCF7.
Conjugation
unconjugated
Clone Number
JE63-11
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA. May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex.
Background References
1. Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K. Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor. Biochem. J. 373:747-757 (2003)
Subcellular Location
Nucleus.
Synonyms
AFP regulator 1 antibody
AFR1 antibody
Alpha fetoprotein regulator 1 antibody
Alpha-fetoprotein regulator 1 antibody
KIAA0854 antibody
RAF antibody
Regulator of AFP antibody
Transcription factor ZHX2 antibody
ZHX2 antibody
ZHX2_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (HA721886) at 1/1,000 dilution.
Lane 1: Neuro-2a cell lysate
Lane 2: COS-1 cell lysate
Lane 3: HeLa cell lysate
Lane 4: C6 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: AGS cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721886, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 92 kDa
Observed band size: 110 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"