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ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]

Usd: 385

Specification

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Catalog# HA721886

ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

ZHX2 Recombinant Rabbit Monoclonal Antibody [JE63-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ZHX2 aa 251-500 / 837.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 92 kDa

Positive Control

Neuro-2a cell lysate, COS-1 cell lysate, HeLa cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, MCF7.

Conjugation

unconjugated

Clone Number

JE63-11

RRID

AB_3096704

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA. May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex.

Background References

1. Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K. Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor. Biochem. J. 373:747-757 (2003)

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9Y6X8 Human

SwissProt: Q8C0C0 Mouse

SwissProt: Q80VX4 Rat

Synonyms

AFP regulator 1 antibody

AFR1 antibody

Alpha fetoprotein regulator 1 antibody

Alpha-fetoprotein regulator 1 antibody

KIAA0854 antibody

RAF antibody

Regulator of AFP antibody

Transcription factor ZHX2 antibody

ZHX2 antibody

ZHX2_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (<a href="/products/HA721886" style="font-weight: bold;text-decoration: underline;">HA721886</a>) at 1/1,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate<br />Lane 2: COS-1 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: PC-12 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: AGS cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721886" style="font-weight: bold;text-decoration: underline;">HA721886</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (HA721886) at 1/1,000 dilution.

    Lane 1: Neuro-2a cell lysate
    Lane 2: COS-1 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: C6 cell lysate
    Lane 5: PC-12 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: AGS cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 110 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721886) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (<a href="/products/HA721886" style="font-weight: bold;text-decoration: underline;">HA721886</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (<a href="/products/HA721886" style="font-weight: bold;text-decoration: underline;">HA721886</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (HA721886) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"