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Zscan4 Mouse Monoclonal Antibody [2-7]

Usd: 350

Specification

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Catalog# EM1701-85

Zscan4 Mouse Monoclonal Antibody [2-7]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Zscan4 Mouse Monoclonal Antibody [2-7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ZSCAN4 aa 1-138 / 433.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 49 kDa

Positive Control

293 cell lysate transfected with Zscan4, 293 cell lysate, HepG2.

Conjugation

unconjugated

Clone Number

2-7

RRID

AB_3073439

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500

Target

Function

Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.

Background References

1. Dan J et al. Zscan4 Inhibits Maintenance DNA Methylation to Facilitate Telomere Elongation in Mouse Embryonic Stem Cells. Cell Rep. 2017 Aug 22;20(8):1936-1949.

2. Zhang B et al.The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1. Nat Commun. 2018 Apr 30;9(1):1723.

Subcellular Location

Nucleus. Telomere.

UNIPROT

SwissProt: Q8NAM6 Human

Synonyms

FLJ35105 antibody

MGC126787 antibody

MGC126789 antibody

Zinc finger and SCAN domain containing 4 antibody

Zinc finger and SCAN domain containing protein 4 antibody

Zinc finger and SCAN domain-containing protein 4 antibody

Zinc finger protein 494 antibody

ZNF 494 antibody

ZNF494 antibody

ZSCA4_HUMAN antibody

Expand

Images

  • Western blot analysis of Zscan4 on different lysates with Rabbit anti-Zscan4 antibody (<a href="/products/EM1701-85" style="font-weight: bold;text-decoration: underline;">EM1701-85</a>) at 1/5,00 dilution.<br /><br />Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 2: Mouse kidney cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/EM1701-85" style="font-weight: bold;text-decoration: underline;">EM1701-85</a>, 1/5,00 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 49 kDa<br />Observed band size: 49 kDa

    Western blot analysis of Zscan4 on different lysates with Rabbit anti-Zscan4 antibody (EM1701-85) at 1/5,00 dilution.

    Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 2: Mouse kidney cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: EM1701-85, 1/5,00 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"