Zscan4 Mouse Monoclonal Antibody [2-7]
Catalog# EM1701-85
Zscan4 Mouse Monoclonal Antibody [2-7]
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WB
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Human
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unconjugated
Overview
Product Name
Zscan4 Mouse Monoclonal Antibody [2-7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human ZSCAN4 aa 1-138 / 433.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 49 kDa
Positive Control
293 cell lysate transfected with Zscan4, 293 cell lysate, HepG2.
Conjugation
unconjugated
Clone Number
2-7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
Background References
1. Dan J et al. Zscan4 Inhibits Maintenance DNA Methylation to Facilitate Telomere Elongation in Mouse Embryonic Stem Cells. Cell Rep. 2017 Aug 22;20(8):1936-1949.
2. Zhang B et al.The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1. Nat Commun. 2018 Apr 30;9(1):1723.
Subcellular Location
Nucleus. Telomere.
UNIPROT
Synonyms
FLJ35105 antibody
MGC126787 antibody
MGC126789 antibody
Zinc finger and SCAN domain containing 4 antibody
Zinc finger and SCAN domain containing protein 4 antibody
Zinc finger and SCAN domain-containing protein 4 antibody
Zinc finger protein 494 antibody
ZNF 494 antibody
ZNF494 antibody
ZSCA4_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Zscan4 on different lysates with Mouse anti-Zscan4 antibody (EM1701-85) at 1/500 dilution.
Lane 1: 293 cell lysate transfected with Zscan4
Lane 2: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-85) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"