AKR1B1 Rabbit Polyclonal Antibody (ER2001-27)
Catalog# ER2001-27
Rabbit Polyclonal to AKR1B1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Rat
-
Mouse
Knock-Out or Knock-Down validated
Western blot analysis of AKR1B1 on different lysates with Rabbit anti-AKR1B1 antibody (ER2001-27) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD AKR1B1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-27) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD AKR1B1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-27) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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