alpha 1 Antitrypsin Recombinant Rabbit Monoclonal Antibody [JF10-03]
Catalog# ET1702-87
alpha 1 Antitrypsin Recombinant Rabbit Monoclonal Antibody [JF10-03]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
alpha 1 Antitrypsin Recombinant Rabbit Monoclonal Antibody [JF10-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human alpha 1 Antitrypsin aa 1-418 / 418.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Molecular Weight
Predicted band size: 47 kDa
Positive Control
Human spleen tissue lysates, MCF-7, Hela, HepG2, human spleen tissue, human liver tissue, human breast carcinoma tissue.
Conjugation
unconjugated
Clone Number
JF10-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
Alpha-1 antitrypsin or α1-antitrypsin (A1AT, α1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. A protease inhibitor, it is also known as alpha1–proteinase inhibitor (A1PI) or alpha1-antiproteinase (A1AP) because it inhibits various proteases (not just trypsin). A1AT is a 52-kDa serpin and, in medicine, it is considered the most prominent serpin; the terms α1-antitrypsin and protease inhibitor (Pi) are often used interchangeably. Most serpins inactivate enzymes by binding to them covalently. These enzymes are released locally in relatively low concentrations where they are immediately cleared by proteins such as A1AT. Besides limiting elastase activity to limit tissue degradation, A1PI also acts to induce locomotion of lymphocytes through tissue including immature T cells through the thymus where immature T cells mature to become immunocompetent T cells that are released into tissue to elevate immune responsiveness. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).
Background References
1. Jacobo SM et al. Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1. Mol Cell Biol 33:1976-90 (2013).
2. Kamimura K et al. Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver. Mol Ther Nucleic Acids 2:e128 (2013).
Sequence Similarity
Belongs to the serpin family.
Tissue Specificity
Ubiquitous. Expressed in leukocytes and plasma.
Post-translational Modification
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.; Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.; (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Subcellular Location
Secreted, Endoplasmic reticulum.
UNIPROT
Synonyms
A1A antibody
A1AT antibody
A1AT_HUMAN antibody
AAT antibody
Alpha 1 antiproteinase antibody
Alpha 1 antitrypsin antibody
Alpha 1 antitrypsin null antibody
Alpha 1 protease inhibitor antibody
Alpha-1 protease inhibitor antibody
Alpha-1-antiproteinase antibody
ExpandImages
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Western blot analysis of alpha 1 Antitrypsin on human spleen tissue lysates with Rabbit anti-alpha 1 Antitrypsin antibody (ET1702-87) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-87) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining alpha 1 Antitrypsin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining alpha 1 Antitrypsin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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