Skip to content

alpha Actinin Recombinant Mouse Monoclonal Antibody [A1G2-R] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA610074

alpha Actinin Recombinant Mouse Monoclonal Antibody [A1G2-R] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

alpha Actinin Recombinant Mouse Monoclonal Antibody [A1G2-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ACTN1 aa 388-619 / 892.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 103 kDa

Positive Control

HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Rat colon tissue lysate, A549 cell lysate, HepG2 cell lysate, MCF7 cell lysate, PC-12 cell lysate, human breast tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

A1G2-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Background References

1. Kunishima S. et. al. ACTN1 mutations cause congenital macrothrombocytopenia. Am. J. Hum. Genet. 92:431-438(2013).

2. Gueguen P. et. al. A missense mutation in the alpha-actinin 1 gene (ACTN1) is the cause of autosomal dominant macrothrombocytopenia in a large French family. PLoS ONE 8:E74728-E74728(2013).

Subcellular Location

Cell membrane, cytoskeleton, Z line, cell junction, ruffle.

UNIPROT

SwissProt: P12814 Human

SwissProt: Q7TPR4 Mouse

SwissProt: Q9Z1P2 Rat

Synonyms

actinin 1 smooth muscle antibody

Actinin alpha 1 antibody

actinin, alpha 1 antibody

ACTN 1 antibody

Actn1 antibody

ACTN1_HUMAN antibody

Alpha Actinin 1 antibody

Alpha actinin cytoskeletal isoform antibody

Alpha-actinin cytoskeletal isoform antibody

Alpha-actinin-1 antibody

Expand

Images

  • This data was developed using <a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 103 kDa<br />Observed band size: 103 kDa<br />Exposure time: 8 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA601194, the same antibody clone in a different buffer formulation.
    Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (HA601194) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Mouse heart tissue lysate (40 µg/Lane)
    Lane 6: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 103 kDa
    Observed band size: 103 kDa
    Exposure time: 8 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601194) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: MCF7 cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: PC-12 cell lysate (20 µg/Lane)<br />Lane 8: C6 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 103 kDa<br />Observed band size: 103 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA601194, the same antibody clone in a different buffer formulation.
    Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (HA601194) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: MCF7 cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: PC-12 cell lysate (20 µg/Lane)
    Lane 8: C6 cell lysate (20 µg/Lane)

    Predicted band size: 103 kDa
    Observed band size: 103 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601194) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha Actinin antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/6,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601194, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-alpha Actinin antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/6,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601194" style="font-weight: bold;text-decoration: underline;">HA601194</a>) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601194, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

alpha Actinin Mouse Monoclonal Antibody [A1G2]

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Rat

Conjugate: unconjugated

metafield image

alpha Actinin Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

alpha Actinin Recombinant Mouse Monoclonal Antibody [A1G2-R]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated