AMACR Rabbit Polyclonal Antibody
Catalog# ER1706-60
AMACR Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
AMACR Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human AMACR aa 1-382 / 382.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, mouse colon tissue, PC-3M.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:1,000
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FC
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1:50-1:100
Target
Function
P504S, also known as AMACR (α-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal β-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy, and/or CBAS4 (congenital bile acid synthesis defect type 4), a disorder characterized by intrahepatic cholestasis, absence of cholic acid from bile, neonatal jaundice and bile duct deficiency. In addition, P504S is overexpressed in prostate cancer and is believed to be functionally important for prostate cancer cell growth.
Background References
1. Amery L et al. Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans. J Lipid Res 41:1752-1759 (2000).
2. Ferdinandusse S et al. Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy. Nat Genet 24:188-191 (2000).
Sequence Similarity
Belongs to the CoA-transferase III family.
Subcellular Location
Mitochondrion. Peroxisome.
Synonyms
2 arylpropionyl CoA epimerase antibody
2 methylacyl CoA racemase antibody
2-methylacyl-CoA racemase antibody
Alpha methylacyl CoA racemase antibody
Alpha methylacyl Coenzyme A racemase antibody
Alpha methylacyl-CoA racemase deficiency, included antibody
Alpha-methylacyl-CoA racemase antibody
Amacr antibody
AMACR deficiency, included antibody
AMACR_HUMAN antibody
ExpandImages
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Western blot analysis of AMACR on different lysates with Rabbit anti-AMACR antibody (ER1706-60) at 1/2,000 dilution.
Lane 1: SW480 (Human colorectal zdenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate (15 µg/Lane)
Lane 4: LNCaP (Human prostate cancer cell) cell lysate (15 µg/Lane)
Lane 5: Mouse liver tissue lysate (30 µg/Lane)
Exposure time: 12 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1706-60, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 42 kDa
Observed band size: 40-42 kDa -
ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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AMACR Recombinant Rabbit Monoclonal Antibody [PSH13-55]
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