ATG9A Recombinant Rabbit Monoclonal Antibody [SC67-05] (ET1610-71)
Catalog# ET1610-71
Rabbit Monoclonal to ATG9A
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: Wild-type NCI-H1299 whole cell lysate (20 µg).
Lane 2: ATG9A knockout NCI-H1299 whole cell lysate (20 µg).
ET1610-71 was shown to specifically react with ATG9A in wild-type NCI-H1299 cells. No band was observed when ATG9A knockout sample was tested. Wild-type and ATG9A knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-71, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ATG9A Recombinant Rabbit Monoclonal Antibody [SC67-05] (ET1610-71)
Immunogen
Synthetic peptide within human atg9a aa 799-839 / 839.
Host
Rabbit
Positive Control
NCI-H1299 cell lysate, Neuro-2a cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC67-05
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 94 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ATG9A
SYNONYMS
APG9 autophagy 9-like 1 antibody;APG9 like 1 antibody;APG9-like 1 antibody;APG9L1 antibody;ATG9 antibody;ATG9 autophagy related 9 homolog A antibody;ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody;ATG9A antibody;ATG9A_HUMAN antibody;Autophagy 9-like 1 protein antibody;Autophagy related protein 9A antibody;Autophagy-related protein 9A antibody;mATG9 antibody;MGD3208 antibody;OTTHUMP00000206046 antibody;OTTHUMP00000206048 antibody;OTTHUMP00000206049 antibody;OTTHUMP00000206062 antibody
SEQUENCE SIMILARITIES
Belongs to the ATG9 family.
SUBCELLULAR LOCATION
Cytoplasmic vesicle, Golgi apparatus, Late endosome membrane, Endoplasmic reticulum membrane.
FUNCTION
Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg9a (autophagy-related protein 9A), also known as APG9-like 1, is a 839 amino acid, multi-pass membrane protein that localizes to the pre-autophagosomal structure (PAS). Isolation membranes are suggested to originate from the PAS, enwrapping cytoplasmic components to form a double membrane autophagosome, which then fuses with the vacuole. Ubiquitously expressed in human adult tissues, Atg9a cycles between the Golgi and endosomes and, with the autophagosome-specific marker LC3, plays a critical role in starvation-induced autophagosome formation. Three isoforms of Atg9a exist as a result of alternative splicing events.
CITATIONS
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Yuan Yujia,et al
Autophagy-deficient macrophages exacerbate cisplatin-induced mitochondrial dysfunction and kidney injury via miR-195a-5p-SIRT3 axis
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WB
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Mouse
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Citation
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