B Raf Recombinant Rabbit Monoclonal Antibody [SU34-04] (ET1608-36)
Catalog# ET1608-36
Rabbit Monoclonal to B Raf
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WB
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: A549-WT cell lysate
Lane 2: A549-KD B Raf cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
B Raf Recombinant Rabbit Monoclonal Antibody [SU34-04] (ET1608-36)
Immunogen
Synthetic peptide within human b raf aa 60-100.
Host
Rabbit
Positive Control
Rat brain tissue, human placenta tissue, human pancreas tissue, mouse fallopian tube tissue, mouse brain tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU34-04
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 84 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
B Raf
SYNONYMS
FLJ95109 antibody;94 kDa B raf protein antibody;B raf 1 antibody;B Raf proto oncogene serine threonine protein kinase antibody;B Raf proto oncogene, serine/threonine kinase antibody;B RAF1 antibody;B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody;BRAF 1 antibody;BRAF antibody;BRAF_HUMAN antibody;BRAF1 antibody;cRmil antibody;MGC126806 antibody;MGC138284 antibody;Murine sarcoma viral (v-raf) oncogene homolog B1 antibody;Murine sarcoma viral v raf oncogene homolog B1 antibody;NS7 antibody;Oncogen BRAF antibody;oncogene BRAF1 antibody;p94 antibody;Proto-oncogene B-Raf antibody;Proto-oncogene c-Rmil antibody;RAFB 1 antibody;RAFB1 antibody;RMIL antibody;Serine/threonine-protein kinase B-raf antibody;v raf murine sarcoma viral oncogene homolog B antibody;v raf murine sarcoma viral oncogene homolog B1 antibody;v-Raf murine sarcoma viral oncogene homolog B1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
TISSUE SPECIFICITY
Brain and testis.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-365 by SGK1 inhibits its activity.; Methylation at Arg-671 decreases stability and kinase activity.; Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm, Cell membrane.
FUNCTION
BRAF is a human gene that encodes a protein called B-Raf. The gene is also referred to as proto-oncogene B-Raf and v-Raf murine sarcoma viral oncogene homolog B, while the protein is more formally known as serine/threonine-protein kinase B-Raf. The B-Raf protein is involved in sending signals inside cells which are involved in directing cell growth. In 2002, it was shown to be mutated in some human cancers. Certain other inherited BRAF mutations cause birth defects.
CITATIONS
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Dendritic Cell-Derived Exosomes Driven Drug Co-Delivery Biomimetic Nanosystem for Effective Combination of Malignant Melanoma Immunotherapy and Gene Therapy
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WB
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Mouse
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Citation
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Jing Zhang;Jin-Shui Zhu
Network pharmacology-based identification of the antitumor effects of taraxasterol in gastric cancer
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IHC
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Mouse
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Citation
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