Skip to content

beta 2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1608-45

beta 2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

beta 2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal human beta 2 Microglobulin.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 14 kDa

Positive Control

Mouse spleen tissue lysate, mouse liver tissue lysate, rat spleen tissue lysate, rat liver tissue lysate, Raji cell lysate, U937 cell lysate, Hela, A431.

Conjugation

unconjugated

Clone Number

SU35-05

RRID

AB_3069806

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

Target

Function

β2 microglobulin also known as B2M is a component of MHC class I molecules, MHC class I molecules have α1, α2, and α3 proteins which are present on all nucleated cells (excludes red blood cells). In humans, the β2 microglobulin protein is encoded by the B2M gene. β2 microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 (5 genes in humans), MR1, the neonatal Fc receptor (FcRn), and Qa-1 (a form of alloantigen). Nevertheless, the β2 microglobulin gene is outside of the MHC (HLA) locus, on a different chromosome. An additional function is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the basolateral membrane of enterocytes and cell membrane of macrophages for degradation resulting in decreased iron uptake from food and decreased iron release from recycled red blood cells in the MPS (mononuclear phagocyte system) respectively. Loss of this function causes iron excess and hemochromatosis. In cytomegalovirus infection, a viral protein binds to β2 microglobulin, preventing assembly of MHC class I molecules and their transport to the plasma membrane.

Background References

1. Zhao Y et al. beta(2)-Microglobulin coaggregates with Abeta and contributes to amyloid pathology and cognitive deficits in Alzheimer\'s disease model mice. Nat Neurosci. 2023 Jul

2. Wang C et al. The immune-related role of beta-2-microglobulin in melanoma. Front Oncol. 2022 Aug

Sequence Similarity

Belongs to the beta-2-microglobulin family.

Post-translational Modification

Glycation of Ile-21 is observed in long-term hemodialysis patients.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P61769 Human

SwissProt: P01887 Mouse

SwissProt: P07151 Rat

Synonyms

B2M antibody

B2MG_HUMAN antibody

Beta 2 microglobin antibody

Beta 2 microglobulin antibody

Beta 2 microglobulin precursor antibody

Beta chain of mhc class 1 proteins antibody

Beta chain of MHC class I molecules antibody

Beta-2-microglobulin form pI 5.3 antibody

CDABP0092 antibody

Hdcma22p antibody

Expand

Images

  • Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat spleen tissue lysate<br />Lane 4: Rat liver tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 12 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat spleen tissue lysate
    Lane 4: Rat liver tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 12 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-beta 2 Microglobulin KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa<br /><br />Exposure time: 62 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-beta 2 Microglobulin KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

    Exposure time: 62 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/500 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: U937 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 2 minutes;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/500 dilution.

    Lane 1: Raji cell lysate
    Lane 2: U937 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa

    Exposure time: 2 minutes;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining of beta 2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of beta 2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of beta 2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of beta 2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

beta 2 Microglobulin Rabbit Polyclonal Antibody

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated