beta Amyloid Recombinant Rabbit Monoclonal Antibody [PSH08-19]
Catalog# HA722962
beta Amyloid Recombinant Rabbit Monoclonal Antibody [PSH08-19]
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WB
-
IHC-P
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IF-Tissue
-
Dot Blot
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Human
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Mouse
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unconjugated
Overview
Product Name
beta Amyloid Recombinant Rabbit Monoclonal Antibody [PSH08-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human beta Amyloid aa 661-710.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Tissue, Dot Blot
Target Molecular Weight
Predicted band size: 87 kDa
Positive Control
APP/PS1, 6-month mouse of AD brain tissue.
Conjugation
unconjugated
Clone Number
PSH08-19
Reactivity Data
| WB | IHC-P | IF-Tissue | Dot Blot | |
|---|---|---|---|---|
| Human |
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| Mouse |
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:2,000
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IHC-P
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1:1,000
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IF-Tissue
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1:50
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Dot Blot
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1:2,000
Target
Function
Amyloid-beta precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. It functions as a cell surface receptor and has been implicated as a regulator of synapse formation, neural plasticity, antimicrobial activity, and iron export. It is coded for by the gene APP and regulated by substrate presentation. APP is best known as the precursor molecule whose proteolysis generates amyloid beta (Aβ), a polypeptide containing 37 to 49 amino acid residues, whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients.
Background References
1. Ono K et al. Aggregation and structure of amyloid beta-protein. Neurochem Int. 2021 Dec
2. Yagi-Utsumi M et al. Conformational Variability of Amyloid-beta and the Morphological Diversity of Its Aggregates. Molecules. 2022 Jul
Subcellular Location
Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.
Synonyms
A4 antibody
A4_HUMAN antibody
AAA antibody
ABETA antibody
ABPP antibody
AICD-50 antibody
AICD-57 antibody
AICD-59 antibody
AID(50) antibody
AID(57) antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid antibody (HA722962) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722962) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid antibody (HA722962) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722962) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid with Rabbit anti-beta Amyloid antibody (HA722962) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722962, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Immunofluorescence analysis of paraffin-embedded mouse brain tissue (negative control) labeling beta Amyloid with Rabbit anti-beta Amyloid antibody (HA722962) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722962, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Dot blot analysis of beta Amyloid on different proteins with Rabbit anti-beta Amyloid antibody (HA722962) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.
Lane 1: Human Aβ37 full length peptide
Lane 2: Human Aβ38 full length peptide
Lane 3: Human Aβ39 full length peptide
Lane 4: Human Aβ40 full length peptide
Lane 5: Human Aβ41 full length peptide
Lane 6: Human Aβ42 full length peptide
Lane 7: Human Aβ43 full length peptide
Lane 8: Mouse Aβ42 full length peptide
Proteins loading: 100ng, 50ng;
Blocking and dilution buffer: 5% NFDM/TBST;
Exposure time: 3 minutes; ECL: K1801.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"