Beta glucuronidase Recombinant Rabbit Monoclonal Antibody [JE54-34]
Catalog# ET7110-66
Beta glucuronidase Recombinant Rabbit Monoclonal Antibody [JE54-34]
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WB
-
IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Beta glucuronidase Recombinant Rabbit Monoclonal Antibody [JE54-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human beta glucuronidase aa 1-160 / 651.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
75 kDa
Positive Control
U937 cell lysate, human lung tissue lysate, THP-1 cell lysates, human prostate carcinoma tissue, human kidney tissue, THP-1.
Conjugation
unconjugated
Clone Number
JE54-34
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
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FC
-
1:50-1:100
Target
Function
Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates.Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. Human β-glucuronidase is located in the lysosome. n the gut, brush border β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption. Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB gene in humans and by the uidA gene in bacteria. Deficiencies in β-glucuronidase result in the autosomal recessive inherited metabolic disease known as Sly syndrome or Mucopolysaccharidosis VII. A deficiency in this enzyme results in the build-up of non-hydrolyzed mucopolysaccharides in the patient. This disease can be extremely debilitating for the patient or can result in hydrops fetalis prior to birth.
Background References
1. Dubot P. et. al. First Report of a Patient with MPS Type VII, Due to Novel Mutations in GUSB, Who Underwent Enzyme Replacement and Then Hematopoietic Stem Cell Transplantation. Int J Mol Sci. 2019 Oct.
2. Bonecker S. et. al. Is the BCR-ABL/GUSB transcript level at diagnosis an early predictive marker for chronic myeloid leukemia patients treated with imatinib? Rev Bras Hematol Hemoter. 2015 Mar-Apr.
Sequence Similarity
Belongs to the glycosyl hydrolase 2 family.
Post-translational Modification
N-linked glycosylated with 3 to 4 oligosaccharide chains.
Subcellular Location
Lysosome.
UNIPROT
Synonyms
Ac2-223 antibody
asd antibody
Beta G1 antibody
Beta glucuronidase antibody
Beta-G1 antibody
Beta-glucuronidase antibody
BG antibody
BGLR antibody
BGLR_HUMAN antibody
FLJ39445 antibody
ExpandImages
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Western blot analysis of Beta glucuronidase on different lysates with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/500 dilution.
Lane 1: U937 cell lysate
Lane 2: Human lung tissue lysate(20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-66) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Beta glucuronidase on THP-1 cell lysates with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-66) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-66) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-66) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Beta glucuronidase was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"