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c-Myc Mouse Monoclonal Antibody [6G6]

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Catalog# RT1149

c-Myc Mouse Monoclonal Antibody [6G6]

Application

  • WB

  • IP

  • IF

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

c-Myc Mouse Monoclonal Antibody [6G6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

peptide

Species Reactivity

Human, Mouse, Rat, Monkey

Validated Applications

WB, IP, IF, IHC-P

Molecular Weight

67kDa

Positive Control

Jurkat, COS

Conjugation

unconjugated

Clone Number

6G6

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃

Storage Buffer

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100-1:1,000

  • IP

  • 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

  • IF

  • 1:50-1:500

  • IHC-P

  • 1:50-1:500 FCM: 1 μg per 1 x 106 cells

Target

Function

C-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

Background References

1. Méndez-Vidal, C., et al. 2013. PTTG2 silencing results in induction of epithelial-to-mesenchymal transition and apoptosis. Cell Death Dis. 4: e530.

2. de la Puerta, M.L., et al. 2013. The autoimmunity risk variant LYP-W620 cooperates with CSK in the regulation of TCR signaling. PLoS ONE 8: e54569

Subcellular Location

Nucleus

UNIPROT

SwissProt: P01106 Human

Synonyms

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MGC105490 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Expand

Images

  • c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining.

    c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining.

  • c-Myc Antibody: RT1149. Western blot analysis of c-Myc expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.

    c-Myc Antibody: RT1149. Western blot analysis of c-Myc expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.

  • c-Myc Antibody AF680: <a href="/products/RT1149" style="font-weight: bold;text-decoration: underline;">RT1149</a> AF680. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz&reg; Blocking Reagent: sc-516214.

    c-Myc Antibody AF680: RT1149 AF680. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214.

  • c-Myc Antibody: RT1149. Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.

    c-Myc Antibody: RT1149. Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.

  • c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.

    c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.

  • c-Myc Antibody: RT1149. Near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz&reg; Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.

    c-Myc Antibody: RT1149. Near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.

  • c-Myc Antibody AF790: <a href="/products/RT1149" style="font-weight: bold;text-decoration: underline;">RT1149</a> AF790. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz&reg; Blocking Reagent: sc-516214.

    c-Myc Antibody AF790: RT1149 AF790. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214.

  • c-Myc Antibody HRP: <a href="/products/RT1149" style="font-weight: bold;text-decoration: underline;">RT1149</a> HRP. Direct western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B).

    c-Myc Antibody HRP: RT1149 HRP. Direct western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B).

  • c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells and nuclear and cytoplasmic staining of lymphoid cells.

    c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells and nuclear and cytoplasmic staining of lymphoid cells.

  • c-Myc Antibody: RT1149. Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.

    c-Myc Antibody: RT1149. Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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