Calreticulin Recombinant Rabbit Monoclonal Antibody [SU37-03] (ET1608-60)
Catalog# ET1608-60
Rabbit Monoclonal to Calreticulin
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HL-60 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: C6 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-60) at 1/50,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Calreticulin Recombinant Rabbit Monoclonal Antibody [SU37-03] (ET1608-60)
Immunogen
Synthetic peptide within human calreticulin aa 40-89 / 417.
Host
Rabbit
Positive Control
HepG2 cell lysate, HeLa cell lysate, HL-60 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Hela, NIH/3T3 cell lysate, NIH/3T3, human liver tissue, human liver carcinoma tissue, human kidney tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU37-03
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:50,000
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IF-Cell
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1:2,000
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IHC-P
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1:50
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FC
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1:2,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Calreticulin
SYNONYMS
Autoantigen RO antibody;CALR antibody;CALR protein antibody;CALR_HUMAN antibody;Calregulin antibody;Calreticulin antibody;cC1qR antibody;CRP55 antibody;CRT antibody;CRTC antibody;Endoplasmic reticulum resident protein 60 antibody;Epididymis secretory sperm binding protein Li 99n antibody;ERp60 antibody;FLJ26680 antibody;grp60 antibody;HACBP antibody;HEL S 99n antibody;RO antibody;Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody;Sicca syndrome antigen A antibody;SSA antibody
SEQUENCE SIMILARITIES
Belongs to the calreticulin family.
SUBCELLULAR LOCATION
Endoplasmic reticulum lumen, Cytoplasm, Secreted, Cell surface, Sarcoplasmic reticulum lumen, nucleus.
FUNCTION
Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
CITATIONS
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Rui Guo,et al
A biomimetic solution, albumin–doxorubicin molecular complex, targeting tumor and tumor-draining lymph nodes
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IHC
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Mouse
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Citation
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Wang Ya,et al
Bispecific Nanobody-Aptamer Conjugates for Enhanced Cancer Therapy in Solid Tumors
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FC
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Human
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Mouse
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Citation
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Wang, X., Wen, Y., Dong, J., Cao, C., & Yuan, S.
Systematic In-Depth Proteomic Analysis of Mitochondria-Associated Endoplasmic Reticulum Membranes in Mouse and Human Testes
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WB
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Human
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Mouse
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Citation
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Minglu Zhou,et al
Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition
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FC
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Mouse
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Citation
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Xueyan Hou,et al
Triggering Pyroptosis by Doxorubicin-Loaded Multifunctional Nanoparticles in Combination with Decitabine for Breast Cancer Chemoimmunotherapy
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IF
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human
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Citation
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