Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] (ET1610-95)

UNIPROT #

P55211

PROTEIN NAME

Caspase-9

SYNONYMS

Caspase9;APAF-3 antibody;APAF3 antibody;Apoptosis related cysteine peptidase antibody;Apoptotic protease Mch-6 antibody;Apoptotic protease-activating factor 3 antibody;CASP-9 antibody;CASP9 antibody;CASP9_HUMAN antibody;Caspase 9 apoptosis related cysteine peptidase antibody;Caspase 9 Dominant Negative antibody;Caspase 9c antibody;Caspase-9 antibody;Caspase-9 subunit p10 antibody;ICE LAP6 antibody;ICE like apoptotic protease 6 antibody;ICE-LAP6 antibody;ICE-like apoptotic protease 6 antibody;MCH6 antibody;PPP1R56 antibody;protein phosphatase 1, regulatory subunit 56 antibody;RNCASP9 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

DEVELOPMENTAL STAGE

Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

POST-TRANSLATIONAL MODIFICATION

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

SUBCELLULAR LOCATION

Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.

FUNCTION

Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.