CD21 Recombinant Rabbit Monoclonal Antibody [SC0681] (ET1610-61)
Catalog# ET1610-61
Rabbit Monoclonal to CD21
Tested for
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WB
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IF-Cell
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IHC-P
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IP
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FC
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (ET1610-61) at 1/5,000 dilution.
Lane 1: Raji cell lysate
Lane 2: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-61) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-61) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.