CDKN2A/p16INK4a Rabbit Polyclonal Antibody (ER1803-53)
Catalog# ER1803-53
Rabbit Polyclonal to CDKN2A/p16INK4a
Tested for
-
WB
-
IF-Cell
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IHC-P
-
FC
Reacts with
-
Human
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of CDKN2A/p16INK4a on different lysates with Rabbit anti-CDKN2A/p16INK4a antibody (ER1803-53) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
