CLEC4F Mouse Monoclonal Antibody [10-A6-1]
Catalog# EM1901-63
CLEC4F Mouse Monoclonal Antibody [10-A6-1]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
CLEC4F Mouse Monoclonal Antibody [10-A6-1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CLEC4F aa 93-426 / 589.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 66 kDa
Positive Control
SH-SY5Y cell lysates, SH-SY5Y.
Conjugation
unconjugated
Clone Number
10-A6-1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
CLEC4F, a member of C-type lectin, was first purified from rat liver extract with high binding affinity to fucose, galactose (Gal), N-acetylgalactosamine (GalNAc), and un-sialylated glucosphingolipids with GalNAc or Gal terminus. CLEC4F is a heavily glycosylated membrane protein co-expressed with F4/80 on Kupffer cells. In contrast to F4/80, CLEC4F is detectable in fetal livers at embryonic day 11.5 (E11.5) but not in yolk sac, suggesting the expression of CLEC4F is induced as cells migrate from yolk cells to the liver. Even though CLEC4F is not detectable in tissues outside liver, both residential Kupffer cells and infiltrating mononuclear cells surrounding liver abscesses are CLEC4F-positive upon Listeria monocytogenes (L. monocytogenes) infection. While CLEC4F has strong binding to Gal and GalNAc, terminal fucosylation inhibits CLEC4F recognition to several glycans such as Fucosyl GM1, Globo H, Bb3∼4 and other fucosyl-glycans. Moreover, CLEC4F interacts with alpha-galactosylceramide (α-GalCer) in a calcium-dependent manner and participates in the presentation of α-GalCer to natural killer T (NKT) cells. This suggests that CLEC4F is a C-type lectin with diverse binding specificity expressed on residential Kupffer cells and infiltrating monocytes in the liver, and may play an important role to modulate glycolipids presentation on Kupffer cells.
Background References
1. Yang CY. et. al. CLEC4F is an inducible C-type lectin in F4/80-positive cells and is involved in alpha-galactosylceramide presentation in liver. PLoS One. 2013 Jun 6;8(6):e65070.
Subcellular Location
Membrane.
UNIPROT
Synonyms
C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 13 antibody
C-type lectin 13 antibody
C-type lectin domain family 4 member F antibody
C-type lectin superfamily member 13 antibody
CLC4F_HUMAN antibody
Clec4f antibody
CLECSF13 antibody
KCLR antibody
Images
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Western blot analysis of CLEC4F on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of CLEC4F was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"