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Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03] (ET1608-27)

Price: Regular price $385.00

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Catalog# ET1608-27

Rabbit Monoclonal to Cyclin B1

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reacts with

  • Human

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03] (ET1608-27)

Immunogen

Synthetic peptide within human cyclin b1 aa 384-433 / 433.

Host

Rabbit

Positive Control

HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, human tonsil tissue, human colon carcinoma tissue, human lymph nodes tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU33-03

RRID

AB_3069797

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:400

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 48 kDa

Isotype

IgG

TARGET

UNIPROT #

P14635

PROTEIN NAME

Cyclin B1

SYNONYMS

CCNB 1 antibody;CCNB antibody;ccnb1 antibody;CCNB1_HUMAN antibody;Cyclin B1 antibody;G2 mitotic specific cyclin B1 antibody;G2/mitotic-specific cyclin-B1 antibody

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin AB subfamily.

DEVELOPMENTAL STAGE

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes. Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. The destruction of the nuclear envelope is important because it allows the mitotic spindle to access the chromosomes.

CITATIONS

  • Dechao Yin,et al

    IPCEF1: Expression Patterns, Clinical Correlates and New Target of Papillary Thyroid Carcinoma

  • An oral phenylacrylic acid derivative suppressed hepatic stellate cell activation and ameliorated liver fibrosis by blocking TGF‐β1 signalling

  • The HSP90 inhibitor KW-2478 depletes the malignancy of BCR/ABL and overcomes the imatinib-resistance caused by BCR/ABL amplification

  • Jiang Huangyu,et al

    Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway

  • Chang-hua Zhou,et al

    MMP13 as an effective target of an active trifluoromethyl quinazoline compound against osteosarcoma

  • Cui Hongjuan,et al

    RNF181 promotes ferroptosis by ubiquitinating HMOX1 to inhibit gastric cancer cell proliferation and chemoresistance

  • Astaxanthin Prevents Tuberculosis-Associated Inflammatory Injury by Inhibiting the Caspase 4/11-Gasdermin-Pyroptosis Pathway

  • Eriocalyxin B Inhibits Adipogenesis in 3T3‑L1 Adipocytes by Cell Cycle Arrest

  • Autophagy-dependent cell cycle arrest in esophageal cancer cells exposed to dihydroartemisinin

  • Zhang Ting,et al

    Trifluoromethyl quinoline derivative targets inhibiting HDAC1 for promoting the acetylation of histone in cervical cancer cells

  • Sun Yuliang,et al

    Intelligent drugs based on notch protein remodeling: a defensive targeting strategy for tumor therapy

  • Zhang, X., Deng, D., Cui, D., Liu, Y., He, S., Zhang, H., Xie, Y., Yu, X., Yang, S., Chen, Y., & Su, Z.

    Cholesterol Sulfate Exerts Protective Effect on Pancreatic β-Cells by Regulating β-Cell Mass and Insulin Secretion

  • Identification of the molecular targets and mechanisms of compound mylabris capsules for hepatocellular carcinoma treatment through network pharmacology and bioinformatics analysis

  • Chao Shen

    Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling

  • Yijuan Xing,et al

    Network pharmacology and metabolomics elucidate the underlying effects and mechanisms of maackiain against endometrial cancer

  • Qian Yiming,et al

    Deguelin inhibits the glioblastoma progression through suppressing CCL2/NFκB signaling pathway

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