Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43] (ET1609-75)
Catalog# ET1609-75
Rabbit Monoclonal to Cytokeratin 10
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cytokeratin 10 on different lysates with Rabbit anti-Cytokeratin 10 antibody (ET1609-75) at 1/500 dilution.
Lane 1: Rat skin tissue lysate
Lane 2: Human skin tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-75) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Rat skin tissue lysate
Lane 2: Human skin tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-75) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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