Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06] (ET1610-42)
Catalog# ET1610-42
Rabbit Monoclonal to Cytokeratin 14
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: PANC-1 cell lysate (15 µg/Lane) (negative)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane) (negative)
Lane 4: PC-12 cell lysate (15 µg/Lane) (negative)
Lane 5: Mouse skin tissue lysate (20 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-42) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06] (ET1610-42)
Immunogen
Recombinant protein within mouse cytokeratin 14 aa 250-484 / 484.
Host
Rabbit
Positive Control
A431 cell lysate, mouse skin tissue lysate, B16F1, SW480, HepG2, human cervical carcinoma tissue, human skin tissue, rat skin tissue, mouse skin tissue, mouse prostate tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC65-06
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:100-1:1,500
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 51 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 14
SYNONYMS
CK 14 antibody;CK-14 antibody;ck14 antibody;Cytokeratin 14 antibody;Cytokeratin-14 antibody;Cytokeratin14 antibody;Dowling Meara antibody;EBS3 antibody;EBS4 antibody;Epidermolysis bullosa simplex antibody;K14 antibody;K1C14_HUMAN antibody;Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody;Keratin 14 antibody;Keratin antibody;Keratin type I cytoskeletal 14 antibody;Keratin, type I cytoskeletal 14 antibody;Keratin-14 antibody;Keratin14 antibody;Koebner antibody;Krt 14 antibody;Krt14 antibody;NFJ antibody;OTTHUMP00000164624 antibody;type I cytoskeletal 14 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
POST-TRANSLATIONAL MODIFICATION
A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.; Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. The gene encoding human Cytokeratin 14 maps to chromosome 17q12-21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.
CITATIONS
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Study of pathological processes of meibomian gland dysfunction by in vitro culture airlifting conditions
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IF
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WB
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Rat
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Citation
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Wu, J. J., Zhu, S., Gu, F., Valencak, T. G., Liu, J. X., & Sun, H. Z.
Cross-tissue single-cell transcriptomic landscape reveals the key cell subtypes and their potential roles in the nutrient absorption and metabolism in dairy cattle
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IF
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Dairy cattle
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Citation
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Li Songhang,et al
A bioswitchable delivery system for microRNA therapeutics based on a tetrahedral DNA nanostructure
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WB
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IF
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Mouse
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Citation
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Li, S., Liu, Y., Zhang, T., Lin, S., Shi, S., He, J., Xie, Y., Cai, X., Tian, T., & Lin, Y.
A Tetrahedral Framework DNA-Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti-Aging
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WB
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IF
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Mouse
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Citation
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