Dopamine beta Hydroxylase Recombinant Rabbit Monoclonal Antibody [JE43-56]
Catalog# HA722558
Dopamine beta Hydroxylase Recombinant Rabbit Monoclonal Antibody [JE43-56]
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WB
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Human
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unconjugated
Overview
Product Name
Dopamine beta Hydroxylase Recombinant Rabbit Monoclonal Antibody [JE43-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Dopamine beta Hydroxylase aa 111-260 / 617.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 69 kDa
Positive Control
SH-SY5Y cell lysate.
Conjugation
unconjugated
Clone Number
JE43-56
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Dopamine beta-hydroxylase (DBH), also known as dopamine beta-monooxygenase, is an enzyme (EC 1.14.17.1) that in humans is encoded by the DBH gene. Dopamine is converted to norepinephrine by the enzyme dopamine β-hydroxylase; ascorbic acid serves as a cofactor. The three substrates of the enzyme are dopamine, vitamin C (ascorbate), and O2. The products are norepinephrine, dehydroascorbate, and H2O. DBH is a 290 kDa copper-containing oxygenase consisting of four identical subunits, and its activity requires ascorbate as a cofactor. It is the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine the only known transmitter synthesized inside vesicles. It is expressed in noradrenergic neurons of the central nervous system (i.e. locus coeruleus) and peripheral nervous systems (i.e. sympathetic ganglia), as well as in chromaffin cells of the adrenal medulla.
Background References
1. Dey SK et al. Dopamine beta hydroxylase as a potential drug target to combat hypertension. Expert Opin Investig Drugs. 2020 Sep
2. Li Z et al. Dopamine beta-hydroxylase and its role in regulating the growth and larval metamorphosis in Sinonovacula constricta. Gene. 2020 May
Subcellular Location
Cytoplasmic vesicle, secretory vesicle membrane, secretory vesicle, chromaffin granule membrane.
UNIPROT
Synonyms
dbh antibody
DBM antibody
Dopamine beta hydroxylase antibody
Dopamine beta monooxygenase antibody
Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
Dopamine beta-monooxygenase antibody
DOPO_HUMAN antibody
OTTHUMP00000022501 antibody
Soluble dopamine beta-hydroxylase antibody
Images
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☑ Relative expression (RE)
Western blot analysis of Dopamine beta Hydroxylase on different lysates with Rabbit anti-Dopamine beta Hydroxylase antibody (HA722558) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722558) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"