DOT1L Recombinant Rabbit Monoclonal Antibody [PSH01-57] (HA721700)
Catalog# HA721700
Rabbit Monoclonal to DOT1L
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: HeLa cell lysate
Lane 4: 293T cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 165 kDa
Observed band size: 180 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: HeLa cell lysate
Lane 4: 293T cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 165 kDa
Observed band size: 180 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
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