FAT tumor suppressor homolog 4 Rabbit Polyclonal Antibody
Catalog# 0905-1
FAT tumor suppressor homolog 4 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
FAT tumor suppressor homolog 4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human FAT tumor suppressor homolog 4 aa 2,264-2,313 / 4,981.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 543 kDa
Positive Control
HepG2, Lovo.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500～1:1,000
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IF-Cell
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1:50-1:100
Target
Function
Protocadherin Fat 4 is a single-pass type I membrane protein that widely expressed in fetal brain, infant brain, brain tumor and colorectal cancer. Cadherins are cell-cell interaction molecules. FAT4 may play the role in the regulation of planar cell polarity.
Background References
1. "Comparative integromics on FAT1, FAT2, FAT3 and FAT4."Katoh Y., Katoh M.Int. J. Mol. Med. 18:523-528(2006)
2. "Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A.Cancer Lett. 197:63-68(2003)
Tissue Specificity
Widely expressed. Expressed in fetal brain, infant brain, brain tumor and colorectal cancer.
Subcellular Location
Membrane
UNIPROT
Synonyms
Cadherin family member 14 antibody
Cadherin related family member 11 antibody
CDHF14 antibody
CDHR11 antibody
FAT atypical cadherin 4 antibody
FAT J antibody
FAT tumor suppressor homolog 4 antibody
Fat-like cadherin protein FAT-J antibody
FAT4 antibody
FAT4_HUMAN antibody
ExpandImages
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Western blot analysis on recombinant FAT4 using anti-FAT4 polyclonal antibody.
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Immunocytochemistry analysis of HepG2 cells labeling FAT tumor suppressor homolog 4 with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of Lovo cells labeling FAT tumor suppressor homolog 4 with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"